新型抗癌药甲啶铂在多壁碳纳米管玻碳电极上的电化学及DPV测定研究

目的: 研究新型抗癌药甲啶铂在多壁碳纳米管玻碳电极(MWCNTs/GCE)上的电化学行为,建立DPV法测定实际样品中甲啶铂的含量。 方法: 循环伏安法(CV)和差分脉冲伏安法(DPV)。 结果: 甲啶铂在MWCNTs/GCE上有1对明显的准可逆氧化还原峰,氧化峰电位E_pa 与还原峰电位E_pc 分别为0.5 V、-0.37 V,△E=0.87 V,I_pa /I_pc=1.30。在优化条件下,甲啶铂的DPV氧化峰电流I_pa 与其浓度C在3.01~158.00 μg·mL^-1 范围内呈良好的线性关系:I_pa=1.735C-0.0107(r=0.9997,n=9)。检出限为0.64 μg·mL^-1。样品测定平均加标回收率为100.4%,RSD为0.8%。 结论: 本方法简便灵敏、准确可靠,可用于甲啶铂的定量测定。

Objective: To investigate the electrochemical behavior of a novel anticancer drug picoplatin on multi-wall carbon nanotubes modified glassy carbon electrode (MWCNTs/GCE),and to establish a DPV method for determination of the picoplatin contents in actual samples. Methods: Cyclic voltammetry (CV) and differential pulse voltammetry (DPV) were applied in the study. Results: A pair of sensitive and quasi-reversible redox peaks of picoplatin was obtained at MWCNTs/GCE with 0.5 V of E_pa and-0.37 V of E_pc,△E=0.87 V,and I_pa /I_pc=1.30.Under the optimized conditions,there was a good linear relationship between the DPV oxidization peak and the concentration of picoplatin in the range of 3.01-158.00 μg·mL^-1.The regression equation was I_pa=1.735C-0.0107(r=0.9997,n=9).The detection limit was 0.64 μg·mL^-1.The corresponding recovery and RSD for standard addition method were 100.4% and 0.8%,respectively. Conclusion: This method is simple,credible and sensitive,which can be used for the quantitative determination of picoplatin.