HPLC测定枣仁安神液中的五味子醇甲

目的: 建立枣仁安神液中五味子醇甲的HPLC含量测定方法。 方法: Supelcosil^TM LC-18-DB ODS (150 mm×4.6 mm,5 μm) 分析柱,流动相为甲醇-水(60: 40),流速1.0 mL·min^-1,检测波长250 nm。 结果: 浓度在0.32~1.28 μg范围内与色谱峰面积呈线性关系(r=0.9999),平均加样回收率96.6%(n=9)。 结论: 方法简便、快速,精密度和稳定性良好,适合于枣仁安神液生产的质量控制。

Objective: To establish an HPLC method for schisandrin in Zaoren Anshen oral solution. Methods: Supelcosil^TM LC-18-DB ODS (150 mm×4.6 mm,5 μm) column was adopted as analytical column with mobile phase of methanol-water (60: 40) at the flow rate of 1.0 mL·min^-1,and detective wavelength was 250 nm. Results: The standard curve was good linear in the range of 0.32-1.28 μg (r=0.9999),and the average recovery was 96.6%(n=9). Conclusion: The HPLC method is convenient,quickly,selective and accurate,which can be used for quality control for Zaoren Anshen oral solution.

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