气相色谱法分析方格星虫多糖的单糖组成
GC analysis of monosaccharides composition of polysaccharide in Sipunculus nudus Linnaeu
分类号：
出版年·卷·期（页码）：2012,32 (8):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
目的: 研究方格星虫多糖的单糖组成。 方法: 采用3 mol·L-1 三氟乙酸将方格星虫多糖水解成单糖,然后与盐酸羟胺进行衍生化反应,采用气相色谱法对其单糖组成进行分析。 结果: 方格星虫多糖的单糖组成主要为阿拉伯糖和葡萄糖,其质量分数分别为7.91%和79.32%。 结论: 此法简单、快速,重现性好,可用于方格星虫多糖的单糖组成分析。
-----英文摘要：---------------------------------------------------------------------------------------
Objective: To determine the monosaccharide compositions of Sipunculus nudus polysaccharide. Methods: Polysaccharide samples were hydrolyzed with 3 mol·L-1 trifluoroacetic acid solution into monosaccharide,and then derivatized with hydroxylamine hydrochloride.The monosaccharide composition of Sipunculus nudus polysaccharide was analyzed by GC. Results: Sipunculus nudus polysaccharide was composed of arabinose and glucose with the mass fractions of 7.91% and 79.32%,respectively. Conclusion: The established method is simple,sensitive and accurate,which can be applied to the monosaccharide compositions analysis for Sipunculus nudus polysaccharide.
-----参考文献：---------------------------------------------------------------------------------------
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