RP-HPLC同时测定大卫颗粒中绿原酸、黄芩苷、_连翘苷、黄芩素和汉黄芩素的含量

目的: 建立RP-HPLC法同时测定大卫颗粒中绿原酸、黄芩苷、连翘苷、黄芩素和汉黄芩素的含量。 方法: 采用Diamonsil C₁₈(200 mm×4.6 mm,5 μm)色谱柱,以乙腈(A)-0.05%磷酸溶液(B)为流动相进行梯度洗脱(0~10 min,10%A→15%A;10~15 min,15%A→25%A;15~25 min,25%A→28%A;25~35 min,28%A→35%A),柱温35℃,流速1.0 mL·min^-1,检测波长230 nm,进样量20 μL。 结果: 绿原酸、黄芩苷、连翘苷、黄芩素和汉黄芩素5个成分质 量浓度分别在2.80~28.0 μg·mL^-1(r=0.9992)、25.71~257.1 μg·mL^-1(r=0.9994)、0.71~7.1 μg·mL^-1(r=0.9995)、0.81~8.1 μg·mL^-1(r=0.9990)、0.50~5.0 μg·mL^-1(r=0.9992)的范围内与峰面积呈良好的线性关系;方法的平均回收率(n=9)分别为100.0%,99.0%,99.0%,99.9%,99.4%。 结论: 所建立的高效液相色谱测定法简便、准确,重复性好,专属性强,可用于大卫颗粒的含量测定质量控制。

Objective: To establish an RP-HPLC method for determination of chlorogenic acid,baicalin,phillyrin,baicalein and wogonin in Dawei granules. Methods: The HPLC separation was achieved on a Diamonsil C₁₈(200 mm× 4.6 mm,5 μm)column at 35℃.The mobile phase consisted of acetonitrile-0.05% phosphoric acid solution with gradient elution(0-10 min,10%A→15%A; 10-15 min,15%A→25%A; 15-25 min,25%A→28%A; 25-35 min,28%A→35%A)at a flow rate of 1.0 mL·min^-1.The UV detection wavelength was set at 230 nm. Results: The linear ranges were 2.80-28.0 μg·mL^-1(r=0.9992),25.71-257.1 μg·mL^-1(r=0.9994),0.71-7.1 μg·mL^-1(r=0.9995),0.81-8.1 μg·mL^-1(r=0.9990),0.50-5.0 μg·mL^-1(r=0.9992),respectively.The average recoveris(n=9)were 100.0%,99.0%,99.0%,99.9% and 99.4%,respectively. Conclusion: The method is simple,accurate and repeatable,and can be used for quality control of Dawei granules.