HPLC法同时测定不同产地及不同采收时间山茱萸中3个_环烯醚萜苷的含量

目的: 采用高效液相色谱法测定不同产地及不同采收期山茱萸中的莫诺苷、马钱苷及獐牙菜苷3个环烯醚萜苷类的含量。 方法: 采用Welchrom-C₁₈ (250 mm×4.6 mm, 5 μm)色谱柱,柱温35℃,以甲醇-0.1%磷酸水溶液为流动相,梯度洗脱,流速1.0 mL·min^-1,检测波长240 nm。 结果: 莫诺苷、马钱苷和獐牙菜苷的进样量分别在12.0~600.0,8.64~432.0,3.72~186.0 μg·mL^-1范围内,与色谱峰面积呈良好线性关系(r=0.9997,n=6);平均回收率(n=6)分别为96.3%,103.4%,98.8%。不同产地的山茱萸中,莫诺苷以西北大学果园采收的含量最高,马钱苷以陕西省丹凤县产的含量最高,獐牙菜苷以西北大学果园采收的含量最高;不同采集时间的西北大学果园山茱萸中莫诺苷、马钱苷和獐牙菜苷的含量范围分别为15.21~50.50, 2.72~8.21,0.30~1.43 mg·g^-1。 结论: 本方法操作简单、快速,重复性好,可同时测定山茱萸中莫诺苷、马钱苷和獐牙菜苷的含量。

Objective: To establish an HPLC method for simultaneous determination of three secoiridoid glucosides(morroniside, loganin and sweroside) in Fructus Corni from different sources and during different harvest periods. Methods: Welchrom C₁₈(250 mm×4.6 mm, 5 μm) column was used and methanol and water(containing 1% phosphoric acid) solution as mobile phase in a gradient elution, the flow rate was 1.0 mL·min^-1, the detection wavelength was at 240 nm and the column temperature at 35℃. Results: The linear ranges of morroniside, loganin and sweroside were 12.0-600.0, 8.64-432.0, 3.72-186.0 μg·mL^-1 μg, respectively(r=0.9997,n=6). The average recoveries(n=6) were 96.3%, 103.4%, 98.8%,respectively. The content of morroniside of sample collected from botanical garden of Northwest University is the highest; The content of loganin of sample collected from Shaanxi Danfeng is the highest; The content of sweroside of sample collected from botanical garden of Northwest University is the highest.The content ranges of morroniside, loganin and sweroside in Fructus Corni from different harvest periods were 15.21-50.50, 2.72-8.21, 0.30-1.43 mg·g^-1. Conclusions: The validated HPLC method has the advantages of precision, reproducibility and reliability, allowing simultaneous determination and comparison of morroniside, loganin and sweroside in Fructus Corni.

1 YU Xi-hua(喻喜华), BI Kai-shun(毕开顺), LI Ze-yun(李泽运),et al.UPLC simultaneous determination of five components in crude and wine treat Fructus Corni(UPLC法同时测定山茱萸生品与酒制品中5个组分的含量).Chin J Pharm Anal(药物分析杂志),2011,31(8):1463
2 ZHAO Xin-feng(赵新峰),SUN Yu-qing(孙毓庆).Micellar electrokinetic chromatographic determination of morroniside and loganin in the traditional Chinese medicine Cornus officinalis Sieb. et Zucc.(胶束电动毛细管色谱法测定山茱萸中莫罗苷和番木鳖苷的含量).Chin J Phamar Anal(药物分析杂志),2007,27(8):1154
3 Wang W,Sun FL,An Y,et al.Morroniside protects human neuroblastoma SH-SY5Y cells against hydrogen peroxide-induced cytotoxicity.Eur J Pharmacol,2009,613(1-3):19
4 Yamabe N,Noh JS,Park CH,et al.Evaluation of loganin,iridoid glycoside from Corni Fructus,on hepatic and renal glucolipotoxicity and inflammation in type 2 diabetic db/db mice.Eur J Pharmacol,2010,648(1-3):179
5 HU Ruo-qi(胡若琪),RAO Xian-yi(饶娴宜).Preventive effect of sweroside on acute hepatic lesion induced by D-galactosamine in rats(獐牙菜苷对D-氨基半乳糖所致急性肝损伤的保护作用).Pharmacol Clin Chin Mater Med(中药药理与临床),1988,4(3):41
6 HE Juan- ni(贺娟妮),LIU Xiao(刘晓),MOU Zhao- li(牟朝丽),et al.Optimization of microwave- assisted extraction with RSM and HPLC determination for three iridoid glycosides in Cornus officinalis(山茱萸中3种环烯醚萜苷的响应曲面优化微波提取及HPLC测定).J Chin Med Mater(中药材),2011,34(7):1118
7 ZHAO Ping(赵平),YAN Run-hong(闫润红),LIU Yang-qing(刘养清),et al.Analysis on contents of loganin and morroniside in cultivated and wild Fructus Corni(栽培与野生山茱萸中马钱苷和莫诺苷含量分析).Chin J Exp Tradit Med Form(中国实验方剂学杂志),2009,15(4):7
8 CHEN Xue-yan(陈学艳),WANG Yan(王艳),LI Jing(李晶),et al.determination of morroniside and loganin in fructus corni by different processes(山茱萸不同炮制品中莫诺苷和马钱苷含量测定).Tianjin J Tradit Chin Med(天津中医药),2010,27(3):254