RP-HPLC法同时测定金铃子汤中的5个主要活性成分

目的: 采用反相高效液相色谱法建立金铃子汤中原阿片碱、黄连碱、巴马汀、延胡索乙素、川楝素含量同时测定的方法。 方法: 采用Kromasil C₁₈(250 mm×4.6 mm,5 μm)色谱柱,流动相为乙腈-0.05%醋酸水溶液(氨水调pH 6.0),梯度洗脱,流速0.8 mL·min^-1,检测波长230 nm,柱温30 ℃。 结果: 原阿片碱、黄连碱、盐酸巴马汀、延胡索乙素、川楝素质量浓度分别在1.76~35.2,1.84~36.8,2.20~44.0,2.24~44.8,8.48~63.6 μg·mL^-1范围内线性良好(r=0.9998~0.9999);平均回收率(n=6)分别为103.5%,101.2%,102.7%,100.9%,97.1%。 结论: 本方法简单快捷,测定结果准确,适用于金铃子汤中原阿片碱、黄连碱、巴马汀、延胡索乙素、川楝素的含量测定,可为金铃子汤的质量控制或中药配伍等方面给与全面、客观的评价。

Objective: To establish the method for determination of protopine, coptisine, palmatine, tetrahydropalmatine and toosendanin in Jinlingzi decoction. Methods: RP-HPLC separation was carried on a Kromasil C₁₈(250 mm×4.6 mm, 5 μm) column with the mobile phase consisting of acetonitrile and 0.05% acetic acid(adjusted to pH 6.0 with ammonia) in a programme of gradient elution. The flow rate was 0.8 mL·min^-1 with a column temperature at 30 ℃, and the detection wavelength was set at 230 nm. Results: The linear ranges of protopine, coptisine, palmatine hydrochloride, tetrahydropalmatine and toosendanin were 1.76-35.2,1.84-36.8,2.20-44.0,2.24-44.8,and 8.48-63.6 μg·mL^-1; The average recoveries(n=6) for the sample preparation of the markers were 103.5%, 101.2%, 102.7%, 100.9%, 97.1%, respectively. Conclusion: The developed method is simple, accurate and feasible, it is a suitable for the determination of protopine, coptisine, palmatine, tetrahydropalmatine, and toosendanin in Jinlingzi decoction. Otherwise it gives a comprehensive and objective evaluation for Jinlingzi decoction.