期刊名称:药物分析杂志 主管单位:中国科学技术协会 主办单位:中国药学会承办:中国食品药品检定研究院 主编:金少鸿 地址:北京天坛西里2号 邮政编码:100050 电话:010-67012819,67058427 电子邮箱:ywfx@nifdc.org.cn 国际标准刊号:ISSN 0254-1793 国内统一刊号:CN 11-2224/R 邮发代号:2-237
|
血必净注射液大鼠体内的药动学研究
Pharmacokinetic studies of Xuebijing injection in rats
作者:
陈向梅1, 王晓雯2, 骆晶3, 贾璞1, 王雪艳4, 肖超妮1, 王世祥1, 刘勤社1,2, 郑晓晖1,4
作者(英文):CHEN Xiang-mei1, WANG Xiao-wen2, LUO Jing3, JIA Pu1, WANG Xue-yan4, XIAO Chao-ni1, WANG Shi-xiang1, LIU Qin-she1,2, ZHENG Xiao-hui1,4
分类号:
出版年·卷·期(页码):2012,32 (5):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------
目的: 建立同时测定丹参素(DSS)、羟基红花黄色素A(HSYA)、芍药苷(PF)、阿魏酸(FA)血浆样品HPLC分析方法,研究血必净注射液中主要有效成分DSS、HSYA、PF、FA在大鼠体内的药代动力学。 方法: 尾静脉注射给药,不同时间采集血样,HPLC测定各成分血药浓度,用DAS 2.0软件拟合药时曲线,对药代动力学参数进行分析。 结果: DSS、HSYA、PF、FA的回收率均大于72%;日内、日间精密度RSD均小于6%;DSS、HSYA、PF、FA的t1/2β分别为(12.206±4.211)min,(54.207±11.516)min,(23.397±8.553)min,(16.482±5.896)min;VF分别为(5.673±1.954)L·kg-1,(0.587±0.375)L·kg-1,(0.866±0.586)L·kg-1,(21.263±2.698)L·kg-1;CL分别为(0.237±0.093)L·min-1,(0.020±0.005) L·min-1,(0.033±0.009)L·min-1,(1.707±0.364) L·min-1。 结论: 该方法简便、准确,可用于血必净注射液的质量控制,适合多组分药代动力学研究,为血必净的临床用药提供依据。
-----英文摘要:---------------------------------------------------------------------------------------
Objective: To establish the HPLC method to analysis danshensu(DSS),hydroxysafflor yellow A(HSYA),paeoniflorin(PF),ferulic acid(FA) in plasma samples,and then study the pharmacokinetic process of Xuebijing injection in rat. Methods: The blood sample was collected at the different time after injected with vein of tail;The concentration of all compounds in the plasma sample were determined by the HPLC.The drug-time curve was fitted with the soft of DAS 2.0 to evaluate the pharmacokinetic parameters. Results: The recovery rate,the RSD of inter- and intra-day precision of all compounds were more than 72%,less than 6%,respectively.The pharmacokinetic parameters of DSS,HSYA,PF and FA were as follows:t1/2β was (12.206±4.211) min,(54.207±11.516) min,(23.397±8.553) min,(16.482±5.896) min,respectively;VF was (5.673±1.954) L·kg-1,(0.587±0.375) L·kg-1,(0.866±0.586) L·kg-1,(21.263±2.698) L·kg-1,respectively;CL was (0.237±0.093) L·min-1,(0.020±0.005) L·min-1,(0.033±0.009) L·min-1,(1.707±0.364) L·min-1,respectively. Conclusion: This method is simple,accurate and successfully applied to the quality control,can provide the basis for clinical treatment and apply to the multi-components in the future.
-----参考文献:---------------------------------------------------------------------------------------
欢迎阅读《药物分析杂志》!您是该文第 1518位读者!
|