牻牛儿苗HPLC指纹图谱及模式识别研究

目的: 研究牻牛儿苗药材的质量控制方法。 方法: 采用HPLC法建立牻牛儿苗药材的HPLC指纹图谱,收集不同产地的23批样品进行测定,并使用聚类分析和主成分分析对指纹图谱进行模式识别研究。 结果: 根据主成分分析和聚类分析结果,筛选14批样品建立指纹图谱共有模式。 结论: 该方法可用于牻牛儿苗质量控制及综合评价。

Objective: To establish a method for quality control of Erodium stephanianum Willd. Methods: An high performance liquid chromatographic method was developed to establish fingerprint of Erodium stephanianum Willd.,and 23 samples of wild and cultivated from various places had been analyzed.Cluster analysis and principal component analysis(PCA) were applied to study HPLC fingerprint and chemical pattern recognition method. Results: 14 samples were selected to establish the common pattern of the fingerprint on the basis of the result of cluster analysis and PCA. Conclusion: The method provides an academic reference for the quality control of Erodium stephanianum Willd..

[1] ChP(中国药典).2005.Vol Ⅰ(一部):263
[2] CHEN Yu-wu(陈玉武),LI Ke-ming(李克明),LI Yao-lan(李药兰),et al.Studies on chemical constituents of Erodium stephanianum Willd.(牻牛儿苗化学成分研究).Chin Tradit Herb Drugs(中草药),2007,38(8):1148
[3] WEI Qun-de(魏群德).Observation of curative effect of Geranium wilfordii Maxim. mixtures on acute pharyngitis(老鹳草合剂治疗急性咽炎的疗效观察).Chin J Integr Tradit Chin Vest Med (中国中西医结合杂志),1998,18(2):12
[4] LUO Yong-jiang(罗永江),HU Zhen-ying(胡振英),CHENG Fu-sheng(程富胜),et al.Research survey of pharmacological action on Geranium wilfordii Maxim.(老鹳草的药理作用研究概况).China Anim Health(中国动物保健),2003,9:24
[5] DU Xiao-ming(杜晓鸣),GUO Yong-si(郭永泗).Geraniin and its antioxidation(老鹳草素及抗氧化作用).World Notes Plant Med(国外医药·植物药分册),1990,5(2):57
[6] Term of Pharmacological Study on Chronic Bronchitis from Xi'an Medical University(西安医学院慢性气管炎药理研究组).Pharmacological study on Erodium stephanianum(绵绵牛的药理研究).Shaanxi Med J(陕西新医药),1978,6:42
[7] FENG Ping-an(冯平安),JIA De-yun(贾德云),LIU Chao(刘超),et al.Study on anti-inflammatory effect of Geranium wilfordii Maxim(老鹳草抗炎作用的研究).Clin J Anhui Tradit Chin Med(安徽中医临床杂志),2003,15(6) :511
[8] LIU Dai-lin(刘岱琳),HU Ying-qing(胡迎庆).Experimental research on crude extract of Geranium wilfordii Maxim. in suppressing generation of nitric oxide in rat macrophages (老鹤草粗提取物抑制大鼠巨噬细胞中一氧化氨发生的实验研究).Med J Chin PAPF(武警医学),2003,14(11):18