巫山淫羊藿饮片炮制前后HPLC指纹图谱研究

目的: 采用高效液相色谱法建立巫山淫羊藿饮片炮制前后的指纹图谱。 方法: 采用Kromasil-C₁₈(4.6 mm×250 mm,5 μm)色谱柱,乙腈和水为流动相,梯度洗脱,流速1.0 mL·min^-1,检测波长270 nm。 结果: 初步建立了巫山淫羊藿生、炙品饮片的HPLC指纹图谱,其精密度、重复性、稳定性试验中共有峰的相对保留时间和相对峰面积的RSD均小于3%。 结论: 该方法简便、准确,重复性好,可应用于巫山淫羊藿生、炙品饮片质量控制。

Objective: To study the HPLC fingerprint of Epimedii Wushanensis Herba before and after processing. Method: Kromasil-C₁₈(4.6 mm×250 mm,5 μm)column was adopted with mobile phase:water and acetonitrile;gradient eluteion at flow rate 1.0 mL·min^-1;The wavelength was set at 270 nm. Result: An HPLC fingerprint of Epimedii Wushanensis Herba after processing was found.The RSD values of relative peak area and relative retention time of common peaks in precision,repeatability and stability are less than 3%. Conclusion: This method is simple,practical and reliable.The method can be used for the qualitative control of Epimedii Wushanensis Herba after processing.

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