HPLC法测定比阿培南二聚物方法研究及其降解途径考察
HPLC determination of biapenem dimer and study on its degradation pathways
分类号：
出版年·卷·期（页码）：2012,32 (3):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
目的: 建立了测定比阿培南二聚物的高效液相色谱法,同时考察二聚物的降解途径。 方法: 采用高效液相色谱法:色谱柱为C18柱,流动相为0.05%三氟乙酸溶液－乙腈(92:8),检测波长为318 nm,流速为1.0 mL·min-1。 结果: 比阿培南溶液浓度在0.000 2~0.076 8 mg·mL-1范围内,溶液浓度与峰面积线性关系良好(r=1.000)。比阿培南二聚物A和二聚物B测定结果的重复性良好,RSD(n=6)分别为2.5%和0.8%。 结论: 本方法操作简便,结果可靠,可以用于比阿培南中二聚物的检测。
-----英文摘要：---------------------------------------------------------------------------------------
Objective: To establish an HPLC analytical method for the determination of biapenem dimer,and study its degradation pathways. Methods: The analytical Column was C18,the mobile phase was 0.05% trifluloracetic acid solution-acetonitrile(92:8),the detective wavelength was 318 nm,and the flow rate was 1.0 mL·min-1. Results: The biapenem had a good linearity in the range of 0.0002-0.0768 mg·mL-1(r=1.000).Repeatability of biapenem dimer A and dimer B was fine,with RSD(n＝6)of 2.5% and 0.8%. Conclusion: The method is simple,rapid and reliable,it is applicable for the quality control of biapenem dimer.
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