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期刊名称：药物分析杂志
主管单位：中国科学技术协会
主办单位：中国药学会承办：中国食品药品检定研究院
主编：金少鸿
地址：北京天坛西里2号
邮政编码：100050
电话：010-67012819,67058427 电子邮箱：ywfx@nifdc.org.cn
国际标准刊号：ISSN 0254-1793
国内统一刊号：CN 11-2224/R
邮发代号：2-237
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霸王花药材HPLC指纹图谱研究
Study on the HPLC fingerprint analysis of Hylocereus undatus raw material
单位(英文)：1. Department of Phytochemistry, China Pharmaceutical University, Nanjing 210009, China;
2. Institute of Traditional Chinese Medicine & Natural Products, Jinan University, Guangzhou 510632, China;
3. Jiangsu Institute for Food & Drug Control, Nanjing 210008, China;
4. Institute of Chinese Medical Sciences, University of Macau, Macau, China
分类号：
出版年·卷·期（页码）：2012,32 (3):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
目的: 建立霸王花药材的HPLC指纹图谱分析方法。 方法: 采用高效液相色谱法对10批霸王花药材进行分析。色谱条件:Phonemenex Luna PFP色谱柱 (250 mm×4.6 mm,5 μm),乙腈-甲醇-0.2%甲酸水溶液梯度洗脱,流速1.0 mL·min-1,检测波长为360 nm,柱温35 ℃。 结果: 10批药材的相似度均在0.92以上,建立了霸王花药材的HPLC指纹图谱,确定了18个共有峰,并利用对照品对照法和LC-MS法对其中的10个共有峰进行了成分指认。 结论: 本法准确、简便,重现性好,可用于霸王花药材的定性鉴别。
-----英文摘要：---------------------------------------------------------------------------------------
Objective: To establish a method of fingerprint analysis on Hylocereus undatus by HPLC. Methods: The analysis was performed on Phonemenex Luna PFP column(250 mm×4.6 mm,5 μm)with a gradient mobile phase of acetonitrile -methanol-0.2% formic acid solution system at flow rate of 1.0 mL·min-1.The detection wavelength was 360 nm.The column temperature was 35 ℃. Results: The similarities of 10 batches samples were over 0.92.The fingerprint of H.undatus was established and 18 common peaks were found.Among them,ten peaks were identified by comparison with the references and LC-MS. Conclusion: The method is accurate,simple,and reproducible,and can be used as a quality control method for H.undatus.
-----参考文献：---------------------------------------------------------------------------------------
[1] Editorial Board of Zhonghua Bencao(《中华本草》编委会).Zhonghua Bencao(中华本草).Vol 6(第六卷).Shanghai(上海):Shanghai Science and Technology Publishers(上海科学技术出版社),2004.865
[2] YI Yan(易衍),WU Xin(巫鑫),WANG Ying(王英),et al.Flavonoids from flower of Hylocereus undatus(霸王花的黄酮类成分研究).J Chin Med Mater(中药材),2011,34(5):712
[3] WU Xin (巫鑫),WANG Ying (王英),HUANG Xiao-jun (黄晓君),et al.Three new glycosides from Hylocereus undatus(霸王花中的三个糖苷类化合物).J Asian Nat Prod Res(亚洲天然产物研究),2011,13(8):728
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