分子印迹固相萃取-液相色谱法测定喘息灵胶囊中盐酸克仑特罗的含量

目的: 建立分子印迹固相萃取-高效液相色谱法测定喘息灵中盐酸克仑特罗含量。 方法: 采用Hypersil BDS C₁₈(250 mm×4.6 mm,5 μm)色谱柱,以0.05 moL·L^-1 NaH₂PO₄(含0.005 moL·L^-1PIC-B6,用磷酸调pH至4.0左右)和甲醇(60∶40)为流动相,检测波长为245 nm,流速为1.0 mL·min^-1;柱温为室温。 结果: 盐酸克仑特罗浓度在0.4~40.0 μg·mL^-1范围内呈良好线性关系(r=0.9998),盐酸克仑特罗的平均回收率为97.8%,(RSD为0.9%,n=9)。 结论: 方法简便,分离效果好,结果准确可靠。

Objective: To establish a method for determination of clenbuterol hydrochloride in Chuanxiling with molecularly imprinted solid phase extraction-high performance liquid chromatography. Methods: The analysis was carried on a Hypersil BDS C₁₈ (250 mm×4.6 mm,5 μm) column;The mobile phase was composed of 0.05 moL·L^-1 NaH₂PO₄ (containing 0.005 moL·L^-1 PIC-B6,adjust the pH to 4.0 with phosphoric acid) and methanol (60∶40);the detection wavelength was 245 nm and the flow rate was 1.0 mL·min^-1;column temperature was at room temperature. Results: The linear range was 0.4-40.0 μg·mL^-1(r=0.9998) for clenbuterol hydrochloride,the average recovery of clenbuterol hydrochloride was 97.8%(RSD=0.9%,n=9). Conclusion: This method is simple,rapid and accurate.

1 Drug Specifications Promulgated by the Ministry of Public Health,P R China.Prescription of Chinese medicine preparations.Vol.5(中华人民共和国卫生部药品标准中药成方制剂第十五册),203
2 TAN Sheng-jian(谭生建),ZHANG Qi-ming(张启明),LIU Gang(刘刚),et al.HPLC determination of ipratropine and clenbuterol hydrochloride in compound yiweibeike aerosol(高效液相色谱法测定复方异维倍克气雾剂中异丙托溴铵和盐酸克仑特罗的含量).Chin J Pharm Anal(药物分析杂志),2005,25(9):1070
3 WANG Xue-qin(王雪芹),LI Yun(李云),WEN Jing-wei(闻京伟).HPLC determination of two components and related substances in ambrocol oral solution(HPLC法测定氨溴特罗口服液中两组分的含量和有关物质).Chin J Pharm Anal(药物分析杂志),2004,24(6):605
4 Ficher L,Muller R,Ekber B,et al.Direct enantioseparation of β-adrenergic blockers using a chiral stationary phase prepared by molecular imprinting.J Am Chem Soc,1991,113:9358
5 LI Geng-sheng(李更生),SANG Hong-xia(尚红霞),LIU Jie(刘婕),et al .Adsorption of clenbuterol molecularly imprinted polymer (克伦特罗分子印迹聚合物吸附性能研究).Chem Bioeng(化学与生物工程),2005,22(6):28
6 MA Jie(马杰).Determination of clenbuterol with molecular imprinting chemiluminescence (分子印迹化学发光法测定瘦肉精的含量).Min Sci Technol (矿业科学技术),2005,33(4):36
7 WANG Pei-long(王培龙),FAN Li(范理),SONG Rong(宋荣),et al.Molecularly imprinted polymer solid phase extraction coupled with gas chromatography-mass spectrometry for the determination of trace clenbuterol hydrochloric in animal urine(动物尿液中盐酸克仑特罗的分子印迹固相萃取-气相色谱-质谱法研究).Chin J Anal Chem(分析化学),2007,35(9):1319