期刊名称:药物分析杂志 主管单位:中国科学技术协会 主办单位:中国药学会承办:中国食品药品检定研究院 主编:金少鸿 地址:北京天坛西里2号 邮政编码:100050 电话:010-67012819,67058427 电子邮箱:ywfx@nifdc.org.cn 国际标准刊号:ISSN 0254-1793 国内统一刊号:CN 11-2224/R 邮发代号:2-237
|
纳米银医用抗菌敷料抗菌性能检测方法的研究
Evaluation of antibacterial activity for medical silver nanoparticles-containing dressing
分类号:
出版年·卷·期(页码):2012,32 (1):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------
目的: 探讨纳米银医用抗菌敷料抗菌性能检测的试验方法。方法: 采用烧瓶振荡法,探讨了摇床转速和培养温度对烧瓶振荡法的影响,并进行了条件设定的优化。同时,利用优化了的烧瓶振荡法对4个批号的纳米银医用抗菌敷料产品的抗菌性能进行检测试验,并与常规抗菌性能检测方法——抑菌环试验法进行对比研究。结果: 在不同转速(100,200,300 r·min-1)和不同温度(25 ℃、37 ℃)的培养条件时,振荡培养前后菌数变化的实验结果显示,在转速200 r·min-1、培养温度25 ℃、作用时间1 h的培养条件下,细菌在振摇前后的菌数差值符合试验限定标准(差值变化不超过10%)。通过采用上述优化的烧瓶振荡法实验条件,检测了4批纳米银医用抗菌敷料,其结果显示具有良好的抗菌性能。其中,对金黄色葡萄球菌的抑菌率分别为97%,80%,80%,90%;对白色念珠菌的抑菌率分别为91%,78%,93%,76%。试验的灵敏性较高,而抑菌环试验结果不能准确地反映出纳米银医用抗菌敷料的抗菌性能。结论: 此优化条件的烧瓶振荡法能够准确检测含纳米银敷料的抗菌性能,为此类敷料产品的抗菌性能检测提供了有效的方法。
-----英文摘要:---------------------------------------------------------------------------------------
Objective: To investigate the method for evaluating antibacterial activity of silver nanoparticles-containing dressing. Methods: Shaking-flask method was used, and the rotational speed and the culture temperature, which were important factors for bacteria growth, were investigated. The refined protocol was used to evaluate the antibacterial activity for 4 batchs of silver nanoparticles-containing dressing; while, the Inhibition Zone Test was used as comparative study.Results: The results of the culture condition(different rotational speed and different culture temperature) selection tests suggested that the condition of 200 r·min-1 rotational speed and 25 ℃, cultured for 1 hour were better for shaking-flask method. This refined method was further used for evaluating antibacterial activity of silver nanoparticles-containing dressing. The results showed that the bacterial inhibition rate were 97%, 80%, 80% and 90% for Staphylcoccus aureus; 91%, 78%, 93% and 76% for Candida albicans in 4 batchs of silver nanoparticles-containing dressing, respectively. In contrast, Inhibition Zone Test could not reflect the antibacterial activity accurately to silver nanoparticles-containing dressing. Conclusion: This refined protocol of shaking-flask method is useful for examining antibacterial activity of silver nanoparticles-containing products accurately, and provided scientific evidences for the establishment of the examination standard of silver nanoparticles-containing products antibacterial performance.
-----参考文献:---------------------------------------------------------------------------------------
欢迎阅读《药物分析杂志》!您是该文第 1263位读者!
|