高效液相色谱法建立银耳多糖特定图谱的研究

目的: 建立银耳多糖特定图谱。 方法: 通过对银耳多糖PMP柱前衍生化,采用高效液相色谱法进行糖组成分析。 结果: 银耳多糖特定图谱由6个共有峰组成,为甘露糖、葡萄糖醛酸、葡萄糖、木糖、岩藻糖以及1个未鉴定出的单糖。 结论: 高效液相色谱法建立多糖的特定图谱,其操作方法简便,分离度高,重复性及稳定性良好,该结果可有效控制银耳多糖的内在质量,同时可作为酸性杂多糖糖组成的测定方法。

Objective: To establish specific chromatogram of Tremella polysaccharides(TP). Methods: The monosaccharide composition of Tremella polysaccharides was determined by HPLC after preparing pre-column derivatization as PMP derivant. Results: There were 6 peaks from the fingerprint which were mannose,glucose acid,glucose,xylose,fucose and an unidentifiable monosaccharide respectively. Conclusion: The HPLC method establishing specific chromatogram is a simple,rapid,sensitive and convenient,which can be applied to the quality control of Tremella polysaccharides. And it also can be used to determine monosaccharide composition of acidic heteropolysaccharides.

[1] NIE Wei(聂伟),ZHANG Yong-xiang(张永祥),ZHOU Jin-huang(周金黄).A review of research on pharmacology of Tremella polysaccharides(银耳多糖的药理学研究概况).Pharm Clin Chin Mater Med(中药药理与临床),2000,16:44
[2] XU Wen-qing(徐文清),WANG Xue-jiao(王雪姣),HUANG Jie(黄洁),et al.Separation and structural features of a neutral heteropolysaccharide A-BTF alkaloid extrace of Tremella fuciformis Berk spores(银耳碱提孢子多糖A-BTF的分离与结构研究).Nat Prod Res Dev(天然产物研究与开发),2007,19:1055
[3] HUANG Xiu-jin(黄秀锦).Comparison study on enzymatic and alkaline extractions,isolation,purification and functional properties of Tremella polysaccharides(银耳多糖的提取、分离、纯化及其功能性质研究).Food Sci(食品科学),2008,29:134
[4] Gao QP,Seljelid R,Chen HQ,et al.Characterization of acidic heteroglycans from Tremella fuciformis Berk with cytokine stimulating activity.Carbohydr Res,1996,288:135
[5] JIANG Rui-zhi(姜瑞芝),CHEN Ying-hong(陈英红),YANG Yong-jie(杨勇杰),et al.GC studies on establish specific(fingerprint) of Tremella fuciformis Berk(气相色谱法建立银耳孢糖特定指纹图谱的探讨).Chin J Pharm Anal(药物分析杂志),2006,26(7):911
[6] MA Ding-yuan(马定远),CHEN Jun(陈军),LI Ping(李萍),et al.Analysis of monosaccharide composition in polysaccharides by pre-column derivatization high performance liquid chromatography(柱前衍生化高效液相色谱法分析多糖中的单糖组成).Chin J Anal Chem(分析化学研究简报),2002,30(6):702
[7] YANG Xing-bin(杨兴斌),ZHAO Yan(赵燕),ZHOU Si-yuan(周四元),et al.Analysis of monosaccharide compositiona in Angelic polysaccharides by pre-column derivatization high performance liquid chromatography(柱前衍生化高效液相色谱法分析当归多糖的单糖组成).Chin J Anal Chem(分析化学研究简报),2005,33(9):1287
[8] LIN Xue(林雪),WANG Zhong-fu(王仲孚),HUANG Ling-juan(黄玲娟),et al.An improved PMP derivatization method for analyzing monosaccharide composition(改进的PMP柱前衍生化方法用于单糖的组成分析).Chem J Chin Univ(高等学校化学学报),2006,27(8):1456