高效液相色谱法测定硫黄中硫的含量
HPLC determination of sulfur(S) in medicinal material Sulfur
分类号:
出版年·卷·期(页码):2012,32 (1):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------
目的: 采用HPLC法测定硫黄中硫的含量。 方法: 采用Fuji C18 5 μm填料自装色谱柱(4.6 mm×200 mm),以甲醇为流动相,流速1.0 mL·min-1,测定波长240 nm。 结果: 硫进样量在0.3962~11.87 μg范围内与峰面积呈良好线性关系(r=0.9999),平均回收率(n=9)为100.2%,精密度、重复性、稳定性均符合要求。 结论: 该方法准确、可靠,可替代滴定法作为硫黄中硫的含量测定方法 。
-----英文摘要:---------------------------------------------------------------------------------------
Objective: To establish an HPLC method for the determination of sulfur(S) in Sulfur. Methods: Fuji C18(4.6 mm×200 mm,5 μm) column was adopted;The mobile phase was methanol at a flow rate of 1.0 mL·min-1;The detection wavelength was 240 nm. Results: The linear range of sulfur(S) was 0.3962-11.87 μg(r=0.9999);The average recovery(n=9) was 100.2%. The methd has good precision,repeatability,stability. Conclusion: The method is accurate and reliable,and can replace the titration for sulfur(S) in medicinal material Sulfur.
-----参考文献:---------------------------------------------------------------------------------------
[1] ZHAO Zhong-jie(赵中杰).Analysis of Minerals Medicine(矿物药分析).Beijing(北京):People’s Medical Publishing House(人民卫生出版社),1991
[2] ChP(中国药典).2010.Vol Ⅰ(一部):315
[3] ChP(中国药典).2010.Vol Ⅱ(二部):63
[4] HU Ying-yuan(胡颖园),HUANG Xiu-ming(黄秀明),LI Chang-fang(李畅方).Analytical method for sulfur in suspension concertrate by HPLC(硫磺悬浮剂的液相色谱分析).Pest Sci Adm(农药科学与管理),2004,25(6):13
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