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期刊名称:药物分析杂志
主管单位:中国科学技术协会
主办单位:中国药学会
承办:中国食品药品检定研究院
主编:金少鸿
地址:北京天坛西里2号
邮政编码:100050
电话:010-67012819,67058427
电子邮箱:ywfx@nifdc.org.cn
国际标准刊号:ISSN 0254-1793
国内统一刊号:CN 11-2224/R
邮发代号:2-237
 

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海南地不容药材定性定量方法研究

Qualitative and quantitative study of Stephania hainanensis H. S. Lo et Y. Tsoong

分类号:
出版年·卷·期(页码):2012,32 (1):0-0
DOI: 10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------

目的: 建立海南地不容药材的定性定量方法。 方法: 采用薄层色谱法对海南地不容药材进行定性鉴别;采用高效液相色谱法测定巴马汀、延胡索乙素、防己诺林碱、粉防己碱的含量,色谱柱为Hypersil-C18(4.6 mm×250 mm,5 μm),流动相为甲醇-0.1%三乙胺体系,梯度洗脱,流速1.0 mL·min-1,柱温25 ℃,检测波长281 nm。参照中国药典2010年版一部所述方法测定水分及总灰分。 结果: 在薄层色谱鉴别中,延胡索乙素和巴马汀斑点分离效果良好,具专属性,可用于海南地不容药材的定性鉴别;高效液相色谱测定中,巴马汀、延胡索乙素、防己诺林碱、粉防己碱进样量分别在0.288~2.88,0.0978~0.815,1.44~9.60,0.116~0.776 μg范围内线性关系良好(r=0.9997~0.9999);平均加样回收率(n=6)分别为97.4%,98.3%,100.8%,97.9%。 结论: 所建立的方法可准确地对海南地不容进行定性、定量分析,用于其质量控制。

-----英文摘要:---------------------------------------------------------------------------------------

Objective: To establish a qualitative and quantitative method for Stephania hainanensis H. S. Lo et Y. Tsoong. Methods: TLC method was used for qualitative identification;An HPLC system was used in the quantitative analysis of four compounds of palmatine,tetrahydropalmatine,fangchinoline and tetrandrine on the analytical column of Hypersil-C18(4.6 mm×250 mm,5 μm),the mobile phase was methanol-0.1% triethylamine with gradient elution at a flow rate of 1.0 mL·min-1,the detection wavelength was 281 nm and the column temperature was 25 ℃. The contents of moisture and ash were determined with the methods in Chinese Pharmacopeia(ChP) 2010. Results: Tetrahydropalmatine and palmatine could be identified by TLC;In the system of HPLC,palmatine,tetrahydropalmatine,fangchinoline and tetrandrine showed good linear relationship in the ranges of 0.288-2.88(r=0.9997),0.0978-0.815(r=0.9999),1.44-9.60(r=0.9998),and 0.116-0.776(r=0.9997)μg,respectively. The average recoveries(n=6) were 97.4%,98.3%,100.8% and 97.9%,respectively. Conclusion: The methods can be used exactly for the qualitative and quantitative analysis of Stephania hainanensis H. S. Lo et Y. Tsoong.

-----参考文献:---------------------------------------------------------------------------------------
[1] YANG He-ming(杨鹤鸣),CHEN Yan(陈嬿).Chemosystematic notes of genus Stephania(Menispermaceae) in China(中国千金藤属植物化学分类概况).Acta Bot Sin(植物学报),1994,36:17
[2] FANG Sheng-ding(方圣鼎),XU Xue-jian(徐学健),CHEN Yan(陈嬿),et al.Study of alkaloids in Stephania(Ⅵ) Alkaloida of Stephania hainanensis H.S.Lo et Y.Tsoong(千金藤生物碱的研究(Ⅵ) 海南地不容中的生物碱).Chin Tradit Herb Drugs(中草药),1987,18(4):2
[3] HUANG Jian-ming(黄建明),GUO Ji-xian(郭济贤),DUAN Geng-li(段更利).Determination of
[7] bio-active alkaloida in Stephania plants by RP-HPLC(RP-HPLC法测定千金藤属植物中7种生物活性生物碱).Acta Pharm Sin(药学学报),1998,33(7):531
[4] ZHANG Yan(张严),XU Yu(徐羽),CUI Jian(崔箭),et al.Determination of active ingredient of drug rehabilitation of Stephania(彝药金不换戒毒作用的有效成分确定).J Cent Univ Natl(Nat Sci Ed)(中央民族大学学报自然科学版),2007,16(4):361
[5] WANG Guang-li(王光丽),WANG Ai-na(王爱娜),QIN Xue-mei(秦雪梅),et al.Study on quality control for Ruan granule(乳安颗粒质量控制的研究).Chin J Pharm Anal(药物分析杂志),2006,26(3):350

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