LC-MS/MS法测定氢溴酸东莨菪碱在大鼠体内的组织分布

目的: 研究氢溴酸东莨菪碱在大鼠体内各组织的分布特点。 方法: 液相色谱分离采用C₁₈(3.0 mm×100 mm,3.5 μm)色谱柱,甲醇-2 mmol·L^-1甲酸铵溶液(40∶ 60,甲酸调pH至3.52)为流动相,流速0.4 mL·min^-1;质谱检测采用ESI离子源,正离子MRM方式检测。采取大鼠灌胃给药,运用液相色谱-质谱/质谱法测定氢溴酸东莨菪碱在大鼠各个组织中的药物浓度。 结果: 氢溴酸东莨菪碱在各个组织中的一定浓度范围内线性关系良好,各个组织中低、中、高浓度氢溴酸东莨菪碱的回收率及精密度均符合方法学要求。氢溴酸东莨菪碱在体内分布广泛,以脾、肝、肺中分布较高。 结论: 本方法简便、准确,灵敏度高,可用于氢溴酸东莨菪碱的体内分析研究。

Objective: To research tissue distribution of scopolamine hydrobromide(SH) in rats. Methods: Chromatographic separation was performed on a C₁₈(3.0 mm×100 mm, 3.5 μm) column with methanol-2 mmol·L^-1ammonium formate (40∶ 60,adjusted to pH 3.52 with formic acid) as the mobile phase at a flow rate of 0.4 mL·min^-1.A trip-quadrupole tandem mass spectrum was applied the electrospray ionization(ESI) source and operated in positive ion multiple reaction monitoring mode. Poured the drug into the stomach of rats, used LC-MS/MS method to determine the SH in tissues of rats in vivo. Results: The linear correlation of SH in different tissues was fine. The recovery and precision were in line with the requirements of methodology. The tissue distributions of SH were wide in rats.The highest level was found in spleen, liver and lung. Conclusion: The LC-MS/MS method of determination of SH in bio-samples is convenient,accurate and sensitive and can be applied in analysis of SH in vivo.

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