三唑醇分子印迹聚合物的制备及在食品检测中的应用

目的: 制备三唑醇分子印迹聚合物(MIP),建立食品中三唑醇残留检测方法。 方法: 以三唑醇为模板分子,α-甲基丙烯酸(MAA)为功能单体,乙二醇二甲基丙烯酸酯(EDMA)为交联剂,采用本体聚合法合成了MIP,以该MIP为固相萃取剂处理样品,并采用高效液相色谱法检测,使用Shim VP-C₁₈色谱柱(150 mm×4.6 mm,5 μm),以甲醇-水(体积比为40:60)作为流动相,检测波长为224 nm。 结果: 三唑醇线性范围为0.1～200 μg·mL^-1,线性相关系数为0.9998,检出限为0.5 μg·g^-1,在2 μg·g^-1和10 μg·g^-1添加水平下,平均回收率在79.3%～86.0%之间,RSD≤2.3%(n=5)。 结论: 该方法灵敏度较高,重现性好,检测快速,是检测食品中三唑醇杀菌剂残留的有效方法。

Objective: To prepare triadimenol molecularly imprinted polymer,and establish a method for determination of triadimenol residues in food. Methods: Molecularly imprinted polymer(MIP) was prepared by the way of bulk polymerization,using triadimenol as template molecule,methacrylate acid(MAA) as functional monomer,ethylene glycol dimethacrylate (EDMA) as crosslinker.MIP was used as solid phase extraction agent to treat samples.Triadimenol residues in the samples were determined by high performance liquid chromatography.Samples were analyzed on Shim VP-C₁₈(150 mm×4.6 mm,5 μm)column,the mobile phase consisted of methanol-water(40:60,v/v),the wavelength was 224 nm. Results: The results showed that the linear range of triadimenol was 0.1-200 μg·mL^-1,linear correlation coefficient was 0.9998;the detection limit was 0.5 μg·g^-1;under 2 μg·g^-1 and 10 μg·g^-1 added levels,the average recoveries were between 79.3%-86.0%,and the relative standard deviations (RSDs) were ≤2.3%(n=5). Conclusion: This method has good sensitivity and reproducibility,is an effective method of detecting triadimenol fungicide residues in food.

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