抗疲劳类保健食品中添加化学成分的快速检测系统研究

目的: 建立抗疲劳类保健食品中非法添加化学成分的快速检测系统。 方法: 在监督现场采用理化反应对大量样品进行快速筛查;在基层药品检测机构采用高效液相色谱法对样品确证;在省级药品检测机构采用液相色谱质谱联用法对阳性样品或影响较大的样品进行仲裁。 结果: 共检测304批样品,发现16批样品非法添加西地那非、他达拉非和氨基他达拉非。以液质联用法测定结果作为依据,2个理化反应快速筛查方法正确率分别为99%和100%,高效液相色谱法的正确率为100%。 结论: 本文从我国监管的实际情况出发,提出的"逐级筛查、分层检测"快速筛查系统,可有效打击抗疲劳类保健食品中非法添加化学成分的行为。

Objective: To establish a rapid detection system of adulteration in the anti-fatigue health food. Methods: Chemical reactions methods were used to rapid screening in the field.HPLC method was used to verify the results.If necessary,LC-MS/MS method was further carried out to arbitrate the positive or important results. Results: batches of samples were determined.16 batches of which contained sildenafil,tadalafil and aminotadalafil.Based on the results of LC-MS/MS,the accuracies of the two chemical reactions screening method were 98% and 100% respectively,and the accuracy of the HPLC method was 100%. Conclusion: According to the current supervision situation of China,this paper proposes the rapid screening system of three levels,which will play an effective role against the adulteration of the health food in the future.