七叶莲提取物中总黄酮和槲皮素的含量测定

目的: 测定七叶莲提取物中总黄酮及槲皮素的含量。 方法: 总黄酮含量测定,以芦丁为对照品,采用分光光度法,于509 nm处测定;槲皮素含量测定,采用高效液相色谱法,色谱柱为Agilent ZORBAXSB-C₁₈(4.6 mm×250 mm,5 μm),流动相为乙腈-0.4%磷酸溶液(36∶ 64),流速1.0 mL·min^-1,检测波长为254 nm,柱温25 ℃。 结果: 总黄酮线性范围为0.0~40.0 μg·mL^-1(r=0.999 9),槲皮素线性范围为0.06~2.40 μg(r=0.999 2);总黄酮和槲皮素的平均回收率分别为100.6%和98.7%。 结论: 建立的方法具有良好的精密度和耐用性,结果准确可靠,可作为本产品的质量控制方法。

Objective: To determine the contents of total flavonoids and quercetin in Schefflera venulosa extracts. Methods: The content of total flavonoids was determined with Vis-UV spectrophotometry;The wavelengh was set at 509 nm;Rutin was used as the control.The separation and quantitation of quercetin was performed on a Agilent ZORBAXSB-C₁₈(4.6 mm × 250 mm,5 μm) column with the mobile phase consisting of acetonitrile - 0.4 % phosphoric acid(36∶ 64) at the flow rate of 1.0 mL·min^-1,the detective wavelength wss 254 nm,and the colunm temperature was 25 ℃. Results: The standard curve was linear in the range of 0.0 - 40.0 μg·mL^-1 for total flavonoids( r=0.9999) and 0.06 - 2.40 μg for quercetin(r =0.9992).The average recoveries of total flavonoids and quercetin were 100.6 % and 98.7 %,respectively. Conclusions: The methods are simple,accurate and with a good reproducibility and can be used to control the quality of Schefflera venulosa extracts.