不同地理居群大蒜药材高效薄层色谱指纹图谱研究

目的: 建立大蒜药材高效薄层色谱指纹图谱,并对不同地理居群大蒜药材的指纹图谱进行比较分析。 方法: 采用高效薄层硅胶G板,以正丁醇-正丙醇-冰醋酸-水(3∶ 1∶ 1∶ 1)为展开剂,以0.2%茚三酮溶液显色后,在日光灯下检视并照相,并在500 nm处对各轨道扫描吸收光谱后获得薄层色谱指纹图谱,经指纹图谱系统解决方案软件生成共有模式,进行相似度分析。 结果: 筛选和优化了高效薄层色谱条件,斑点清晰,分离度好;相似度分析表明,不同地理居群的大蒜药材在药用活性成分相对含量上有不同程度的差异,大致可分为5类。 结论: 建立的高效薄层色谱指纹图谱,为大蒜药材指纹图谱的研究奠定了方法学基础,可以作为不同来源大蒜药材质量控制的重要依据。

Objective: To establish high performance thin layer chromatography(HPTLC) fingerprint of garlic herbs and compare the fingerprints of garlic in different geographic populations. Methods: High performance thin layer silica gel G plates was used,n-butanol-n-propanol-glacial acetic acid-water(3∶ 1∶ 1∶ 1) was developing agent,after being colored by 0.2% ninhydrin,the plates were put under the fluorescent light and taken photos,and then the HPTLC fingerprint was obtained with scanning absorption spectrum of each track at 500 nm,at last,the common model was generated with the Chromap software and similarity analysis was performed. Results: The HPTLC conditions were selected and optimized,the spots were clear and had the better separation.The results of similarity analysis showed that the garlic of different geographical populations had different levels on the relative content of medicinal active ingredients,the total samples could be divided into 5 class. Conclusion: The HPTLC fingerprint has laid the methodological foundation for study on garlic fingerprint,and can be regarded as an important basis of quality control for garlic from different sources.