HPLC法同时测定齿叶白鹃梅叶子中芹菜素-7-O-β-D-新橙皮糖苷和芹菜素-7-O-β-D-葡萄糖苷的含量

目的: 建立同时测定齿叶白鹃梅叶子中芹菜素-7-O-β-D-新橙皮糖苷和芹菜素-7-O-β-D-葡萄糖苷含量的HPLC法。 方法: 采用Topsil C₁₈(5 μm,4.6 mm×250 mm)色谱柱,流动相为乙腈-2%醋酸水溶液(23∶ 77),流速1.0 mL·min^-1,检测波长267 nm。 结果: 芹菜素-7-O-β-D-新橙皮糖苷和芹菜素-7-O-β-D-葡萄糖苷的线性范围分别为1.424~8.544 μg(r=0.9996)和0.17~1.02 μg(r=0.9995);平均回收率(n=6)分别为103.0%(RSD=2.2%)和100.4%(RSD=2.1%)。 结论: 该方法准确、可靠,可用于齿叶白鹃梅叶子的质量控制。

Objective: To develop an HPLC method for determination of the contents of apigenin-7-O-β-D-neospheroside and apigenin-7-O-β-D-glucopyranoside in the leaves of Exochorda serratifolia S.Moore. Methods: Topsil C₁₈(5 μm,4.6 mm×250 mm) column was adopted with a mobile phase of acetonitrile-2% acetic acid(23∶ 77),at the flow rate of 1.0 mL·min^-1.The UV detection was set at 267 nm. Results: The linear ranges of apigenin-7-O-β-D-neospheroside and apigenin-7-O-β-D-glucopyranoside were 1.424-8.544 μg(r=0.9996)和0.17-1.02 μg(r=0.9995),respectively;The average recoveries of apigenin-7-O-β-D-neospheroside and apigenin-7-O-β-D-glucopyranoside were 103.0%(RSD=2.2%) and 100.4%(RSD=2.1%). Conclusion: The method is accurate and reliable,and can be used to the quality control of leaves of Exochorda serratifolia.