顶空气相色谱法测定苦参总碱中三氯甲烷的残留量
Headspace GC determination of residual chloroform in matrine
分类号：
出版年·卷·期（页码）：2011,31 (9):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要：-------------------------------------------------------------------------------------------
目的: 建立苦参总碱中三氯甲烷残留的检测方法。 方法: 采用气相色谱顶空进样法,聚乙二醇(DB-WAX)毛细管柱,FID检测器,柱温采用程序升温,初始温度为60 ℃,保持6 min,然后以15 ℃·min-1升温速率升至180 ℃,保持5 min。 结果: 各个峰的分离效果好,三氯甲烷浓度在12.4~1236.6 μg·mL-1的范围内呈良好的线性关系(r=0.9940),平均回收率为94.5%。 结论: 本方法结果准确、灵敏度高、重复性好,可以作为苦参总碱中三氯甲烷残留的检测方法。
-----英文摘要：---------------------------------------------------------------------------------------
Objective: To establish a method to determine the residual chloroform in matrine. Methods: Using DB-WAX capillary column,FID detector and program temperature.The initial temperature was kept at 60 ℃ for 6 minutes,then the temperature was raised to 180 ℃ at the rate of 15 ℃·min-1 and kept for 5 minutes. Results: All the peaks were completely separated,the calibration curve showed a good linearity with the range of 12.4-1236.6 μg·mL-1(r=0.9940).The average recovery was 94.5%. Conclusion: The method is accurate,sensitive and repetitive to determine residual chloroform in matrine.
-----参考文献：---------------------------------------------------------------------------------------
欢迎阅读《药物分析杂志》！您是该文第 1122位读者！
