HPLC法测定北刘寄奴中木犀草素和芹菜素的含量

目的: 建立同时测定北刘寄奴中木犀草素和芹菜素含量的HPLC方法。 方法: 采用Inertsil ODS-3(4.6 mm×150 mm,5 μm)色谱柱,以乙腈-0.5%磷酸(28∶ 72)为流动相,流速1.0 mL·min^-1,检测波长340 nm,柱温35 ℃。 结果: 木犀草素和芹菜素进样量分别在0.2168~1.951 μg和0.0684~0.615 μg范围内与峰面积呈良好的线性关系(r=0.9999);木犀草素和芹菜素平均加样回收率(n=5)分别为99.7%和99.1%,RSD分别为0.99%和1.5%。 结论: 该方法简便、准确、可靠,可用于中药北刘寄奴中木犀草素和芹菜素含量的测定。

Objective: To develop an HPLC method for content determination of luteolin and apigenin in Siphonostegia chinensis. Methods: The separation was carried out on a Inertsil ODS-3 (4.6 mm×150 mm, 5 μm) column with acetonitrile-0.5% phosphoric acid(28∶ 72) as mobile phase at a flow rate of 1.0 mL·min^-1. The detection wavelength was 340 nm and the column temperature was maintained at 35 ℃. Results: There were good linear relationships between the masses and the peak areas of luteolin and apigenin in the ranges of 0.2168-1.951 μg(r=0.9999) and 0.0684-0.615 μg(r=0.9999),respectively. The average recoveries(n=5) were 99.7% and 99.1%,and RSDs were 0.99% and 1.5%,respectively. Conclusion: This method is simple, precise and reliable for the determination of luteolin and apigenin in Siphonostegia chinensis.