多波长高效液相色谱法同时测定栀子厚朴汤醇提液中6个主要成分的含量

目的: 建立同时测定栀子厚朴汤醇提液中栀子苷、柚皮苷、橙皮苷、新橙皮苷、和厚朴酚与厚朴酚含量的高效液相色谱方法。方法: 采用Hypersil C₁₈(250 mm×4.6 mm,5 μm)色谱柱,以乙腈-水为流动相,梯度洗脱,流速1.0 mL·min^-1,检测波长为238 nm(0~12 min)、283 nm(12~30 min)、294 nm(30~40 min),柱温30 ℃。结果: 栀子苷、柚皮苷、橙皮苷、新橙皮苷、和厚朴酚、厚朴酚的进样量分别在1.03~10.3 μg(r=0.9996)、0.184~1.84 μg(r=0.9996)、0.880~8.80 μg(r=0.9997)、0.478~4.78 μg(r=0.9996)、0.221~2.21 μg(r=0.9998)、0.538~5.38 μg(r=0.9998)范围内与各自峰面积值呈良好线性关系;平均回收率(n=6)分别为99.4%,99.2%,102.4%,100.3%,102.0%,99.8%。结论: 本方法简便、准确、可靠,可用于栀子厚朴汤醇提液的质量控制。

Objective:To establish an HPLC method for simultaneous determination of geniposide,naringin,hesperidin,neohesperidin,honokiol,and magnolol in Zhizihoupu ethanol extraction.Methods: Hypersil C₁₈ colunm(250 mm×4.6 mm,5 μm) was adopted with the mobile phase consisting of acetonitrile-water at a flow rate of 1.0 mL·min^-1, and the detection wavelength was set at 238 nm(0-12 min)、283 nm(12-30 min)、294 nm(30-40 min),and the column temperature was maintained at 30 ℃.Results: The calibration curves of geniposide, naringin, hesperidin, neohesperidin, honokiol, and magnolol were in good linearity over the ranges of 1.03-10.3 μg(r=0.9996), 0.184-1.84 μg(r=0.9996), 0.880-8.80 μg(r=0.9997), 0.478-4.78 μg(r=0.9996), 0.221-2.21 μg(r=0.9998), 0.538-5.38 μg(r=0.9998), respectively. And the average recoveries of the six constituents were 99.4%,99.2%,102.4%,100.3%,102.0% and 99.8%,respectively. Conclusion:The method is simple,accurate and reliable,and can be used for the quality control of Zhizihoupu ethanol extraction.