1酸水解前后甘草中4个黄酮类化合物含量的变化

目的: 建立甘草提取物中4个活性成分甘草苷、异甘草苷、甘草素和异甘草素的测定方法,并评价酸水解法对4个黄酮类化合物含量测定的影响。 方法: 采用高效液相色谱法,分别测定乌拉尔甘草乙醇提取液和酸水解提取液中4个活性成分的含量。色谱柱为Agilent HC-C₁₈(150 mm×4.6 mm,5 μm)柱,流动相为pH 3甲酸溶液-乙腈,梯度洗脱,流速0.8 mL·min^-1,甘草苷和甘草素检测波长为276 nm,异甘草苷和异甘草素的检测波长为370 nm,柱温为25 ℃(室温)。 结果: 乌拉尔甘草乙醇提取液中甘草苷、异甘草苷、异甘草素占原药材的质量百分数分别为1.29%, 0.33%,0.01%,因药材中甘草素含量过低,未检出;酸水解提取液中甘草苷、异甘草苷、甘草素、异甘草素占原药材的质量百分数分别为0.71%,0.32%,0.26%,0.11%。 结论: 酸水解法能显著提高甘草素和异甘草素的含量测定值。

Objective: To develop an HPLC method for the analysis of four flavonoid marker compounds(liquiritin,isoliquiritin,liquiritigenin,isoliquiritigenin) in Glycyrrhiza uralensis Fisch.extracts,and evaluate the influence of acid-hydrolysis method by comparing the content of the four mark components. Methods: The contents of four active components,both of the ethanol extract and the acid-hydroolysis extract were determined by HPLC.The separation was performed on a reversed-phase Agilent HC-C₁₈(150 mm×4.6 mm,5 μm)column by using a gradient elution with mobile phase of water-formic acid(pH 3)and acetonitrile.The assay was carried out at a flow rate of 0.8 mL·min^-1at 25 ℃(room temperature).The detection wavelength for liquiritin and liquiritigenin can be determined at 276 nm while isoliquiritin and isoliquiritigenin at 370 nm. Results: The contents of liquiritin,isoliquiritin and isoliquiritigenin of ethanol extract were separately 1.29%,0.33% and 0.01% comparing with its original medicinal,the content of liquiritigenin was too low to be detected.For the acid-hydrolysis extract,the content of liquiritin,isoliquiritin,liquiritigenin,and isoliquiritigenin were separately 0.71%,0.32%,0.26% and 0.11% comparing with its original medicinal. Conclusion: Acid-hydrolysis method can markedly enhance the measured value of content of liquiritigenin and isoliquiritigenin.