蒲黄-五灵脂配伍对少腹逐瘀汤中芳香酸及萜苷类成分溶出的影响

目的: 建立高效液相色谱法同时测定少腹逐瘀汤、少腹逐瘀汤减去蒲黄-五灵脂药对中绿原酸、咖啡酸、芍药苷、阿魏酸、肉桂酸含量,以评价蒲黄-五灵脂配伍对少腹逐瘀汤中化学成分溶出的影响。 方法: 采用SunFire^TM C₁₈(4.6 mm×250 mm,5 μm)色谱柱,以乙腈-0.5%醋酸水溶液为流动相,进行梯度洗脱,流速0.8 mL·min^-1,二极管阵列检测器,检测波长323 nm、230 nm和277 nm,柱温30 ℃,外标法定量。 结果: HPLC-DAD分析测定结果表明,少腹逐瘀汤全方中咖啡酸、芍药苷、阿魏酸和肉桂酸含量均高于全方减去蒲黄-五灵脂药对,而绿原酸在少腹逐瘀汤全方中含量则低于全方减去蒲黄-五灵脂药对。 结论: 经过蒲黄-五灵脂配伍后可使少腹逐瘀汤全方中某些有效成分的溶出增加,为进一步揭示该方效应物质基础及配伍机理提供一定科学依据。

Objective: To establish an HPLC method for simultaneous determination of aromatic acids and terpenoids in Shaofu Zhuyu decoction and Shaofu Zhuyu decoction minus Puhuang-Wulingzhi drug pair.This method was to evaluate the actions with compatibility of Puhuang-Wulingzhi drug pair in Shaofu Zhuyu decoction. Methods: Five compounds(chlorogenic acid,caffeic acid,peoniflorin,ferulic acid,cinnamic acid)were analyzed simultaneously with a SunFire^TM C₁₈(4.6 mm×250 mm,5 μm)column by gradient elution using acetonitrile-0.5% acetic acid as the mobile phase,the flow rate was 0.8 mL·min^-1,the column was maintained at 30 ℃ and detected with diode array detector(DAD)at 323 nm,230 nm and 277 nm. Results: HPLC-DAD analysis results showed that,the content of caffeic acid,peoniflorin,ferulic acid,cinnamic acid in Shaofu Zhuyu decoction was higher than the party minus the Puhuang-Wulingzhi drug pair;and the content of chlorogenic acid in Shaofu Zhuyu decoction was lower than the party minus the Puhuang-Wulingzhi drug pair. Conclusion: The result in this paper would provide the scientific basis when revealing the effect substance basis and compatibility mechanism of Shaofu Zhuyu decoction.