RP-HPLC同时测定代温灸膏中肉桂酸、桂皮醛、_辣椒碱、二氢辣椒碱及6-姜酚的含量

目的: 建立用高效液相色谱法同时测定代温灸膏中主要药效成分肉桂酸、桂皮醛、辣椒碱、二氢辣椒碱及6-姜酚的含量。 方法: 采用Alltima C₁₈(4.6 mm×250 mm,5 μm)色谱柱,流动相为乙腈-0.1%磷酸,梯度洗脱,流速1 mL·min^-1,检测波长280 nm,柱温30 ℃。 结果: 肉桂酸、桂皮醛、辣椒碱、二氢辣椒碱、6-姜酚浓度分别在5.66~42.45,20.40~153.0,16.20~121.5,10.16~76.20,13.12~98.40 μg·mL范围内与峰面积呈良好的线性关系。上述化合物的回收率和RSD分别为98.2%,1.4%;100.0%,1.0%;99.2%,1.1%;99.2%,1.2%;100.1%,1.0%。 结论: 该方法能准确、快速分析代温灸膏中5个主要成分,灵敏度高,重现性好,可用于控制代温灸膏的质量。

Objective: To establish an RP-HPLC method for the determination of cinnamic acid,cinnamal,capsaicin,dihydrocapsaicin and 6-gingerol in Dai-wen-jiu-gao preparation. Methods: The sample were separated on an Alltima C₁₈ (4.6 mm×250 mm,5 μm) column by gradient elution with the moblie phase of acetonitrile-0.1% phosphoric acid at a flow rate of 1 mL·min^-1.The detective wavelength was set at 280 nm,and column temperature was 30 ℃. Results: The calibration curves for cinnamic acid,cinnamal,capsaicin,dihydrocapsaicin and 6-gingerol revealed linearity over the range of 5.66-42.45,20.40-153.0,16.20-121.5,10.16-76.20,and 13.12-98.40 μg·mL^-1.The recoveries of compounds were 98.19% with RSD of 1.4%,100.0% with RSD of 1.0%,99.20% with RSD of 1.1%,99.15% with RSD of 1.2%,and 100.1% with RSD of 1.0%,respectively. Conclusion: The method is simple,rapid and accurate,which is suitable for the qualitative control of Dai-wen-jiu-gao preparation.