UPLC法同时测定山茱萸生品与酒制品中5个组分的含量

目的: 建立UPLC法同时测定山茱萸中没食子酸、5-羟甲基糠醛、莫诺苷、马钱苷和当药苷的含量。 方法: ACQUITY UPLC^TMHSS C₁₈色谱柱(2.1 mm×100 mm,1.8 μm);流动相:乙腈(A)-0.1%甲酸水(B);梯度洗脱(0~3 min,5%A;3~12 min,5%A→20%A);流速:0.2 mL·min^-1;检测波长:250 nm;柱温:30 ℃。 结果: 在上述条件下,没食子酸、5-羟甲基糠醛、莫诺苷、马钱苷和当药苷的质量浓度分别在10.50~525.2 μg·mL^-1(r=0.9993),10.85~542.5 μg·mL^-1(r=0.9994),13.50~652.4 μg·mL^-1(r=0.9999),11.85-592.6 μg·mL^-1(r=0.9992),5.06~252.8 μg·mL^-1(r=0.9991)范围内与色谱峰面积呈良好的线性关系;低、中、高浓度的平均加样回收率(n=3)均在97.5%~102.5%之间,RSD均小于2.9%。 结论: 该方法简便、快速、准确,重复性好,可用于山茱萸药材的质量控制。

Objective: To establish a method for the determination of gallic acid,5-hydroxymethylfurfural,morrniside,loganin and sweroside in Fructus Corni. Method: The separation was performed on an ACQUITY UPLC^TMHSS C₁₈(2.1×100 mm,1.8 μm) column with a gradient elution(0-3 min,5%A;3-12 min,5%A→20%A) composed of acetonitrile and 0.1% formic acid.The temperature was set at 30 ℃ with flow rate of 0.2 mL·min^-1 and the detective wavelength was set at 250 nm. Results: The linear ranges were 10.50-525.2 μg·mL^-1(r=0.9993),10.85-542.5 μg·mL^-1(r=0.9994),13.50-652.4 μg·mL^-1(r=0.9999),11.85-592.6 μg·mL^-1(r=0.9992),and 5.06-252.8 μg·mL^-1(r=0.9991),respectively.The average recoveries(n=3) of five components were 97.5%-102.5% with RSDs of recoveries were less than 2.9%. Conclusion: The method is simple,rapid,accurate and reproducible for the quality control of Fructus Corni.