反相高效液相色谱法测定维药芜菁中槲皮素的含量

目的: 建立HPLC法测定维药芜菁中槲皮素含量的方法。 方法: 反相高效液相色谱法,色谱条件: Kromasil C₁₈ 柱(5 μm, 250 mm × 4.6 mm);流动相:甲醇 - 0.4% 磷酸(42∶ 58, v/v);检测波长:370 nm。 结果: 测定方法在5.0~50 μg·mL^-1浓度范围内呈良好的线性关系(r = 0.9991),平均回收率为 99.2% (RSD =1.59% , n= 6)。 结论: 方法简便可行、重复性好,可作为芜菁质量评价的方法之一。同时通过本实验测得芜菁中槲皮素的平均含量为203.8μg·g^-1,此数据为进一步开发利用新疆丰富的药物资源提供了参考依据。

Objective: To describe a method for determining Quercetin in Brassica rapa L. by RP-HPLC. Methods: The determination was carried out over a Kromasil C₁₈ column (5 μm, 250 mm × 4.6 mm ) at room temperature(20℃-25℃). The mobile phase was CH₃OH - 0.4％H₃PO₄ (42∶ 58,v/v) with flow rate of 1.0 mL· min^-1, the detection wavelength was 370 nm . Results: There was a linear correlation between the concentration of Quercetin (5.0 -50μg·mL^-1) and the peak area value (r=0.9991), the average recovery was 99.2％ whith the RSD of 1.59 % (n=6). Conclusion: The method is simple,and had good linear relationship. This method can be used to identify and evaluate the quality of Xinjiang Brassica rapa L..