毒性中药蟾酥质量检测方法研究

目的: 建立蟾蜍中多种药效及毒性成分蟾蜍毒素的定性定量检测方法。 方法: 采用液相色谱-质谱/质谱联用方法检测蟾酥中多种蟾蜍毒素成分。 结果: 建立了华蟾毒它灵、蟾蜍灵、华蟾毒配基及脂蟾毒配基的LC-MS/MS检测方法。比较了蟾酥样本的液相色谱-质谱/质谱联用分析数据。对4个不同来源中华大蟾蜍及黑眶蟾蜍的蟾酥样本进行检测,检出华蟾毒它灵、蟾蜍灵、华蟾毒配基及脂蟾毒配基的含量范围为0.05%~5.12%(w/w)。 结论: 液相色谱-质谱/质谱联用方法可用于蟾酥定性定量检测,并为质量评价提供参考。

Objective: To establish quality control method for Venenum Bufonis (Chansu) which was secretion material obtained from Bufo gargarizans Cantor or Bufo melanostictus Schneider.Chansu contains bufadienolide steriods with both therapeutic properties and toxicity. Method: Using LC-MS/MS technique to establish quality control method for Chansu by determining the bufadienolide steriods. Results: Established LC-MS/MS method was used to determine 4 bufadienolide steroids viz.cinobufotalin,bufalin,resibufagenin and cinobufagin that could be found in Chansu.Chansu samples from four different sources of Bufo gargarizans Cantor or Bufo melanostictus Schneider were analysed and the determined content of the 4 different bufadienolide steriods ranged from 0.05% to 5.12% (w/w). Conclusion: This paper developed an LC-MS/MS method for qualitative and quantitative analysis of Chansu,which can be applied to assessing the quality of the herb.