清开灵不同制剂中黄芩苷的含量测定

目的: 统一清开灵软胶囊、泡腾片和口服液中黄芩苷的含量测定方法。 方法: 采用高效液相色谱法,使用Agilent TC-C₁₈(150 mm×4.6 mm,5 μm)色谱柱,流动相为甲醇-水-磷酸(47∶ 53∶ 0.2),流速为1 mL·min^-1,检测波长277 nm。 结果: 黄芩苷的质量浓度在8.928~89.28 μg·mL^-1范围内与峰面积线性关系良好,r=0.9994;精密度、重复性、稳定性及加样回收率结果均能满足分析要求。 结论: 该方法简单可行,为清开灵药品中黄芩苷含量测定方法的统一提供可靠的依据。

Objective: To unified the method for the content determination of baicalin of Qingkailing soft capsules,effervescent tablets and oral liquid. Methods: Baicalin was analyzed on an Agilent TC-C₁₈ (150 mm×4.6 mm,5 μm) column;The mobile phase consisted of methanol-water-phosphoric acid (47∶ 53∶ 0.2) at the flow rate of 1.0 mL·min^-1;The detection wavelength was 277 nm. Results: Baicalin has a good linearity in the range of 8.928-89.28 μg·mL^-1(r=0.9994) with satisfied precision,repetition,stability and recovery. Conclusion: This method is simple,feasible,and can offer the reliable basis for unification of the standards.