HPLC法测定不同来源红花中对香豆酸的含量

目的: 采用高效液相色谱法测定不同来源红花中对香豆酸含量。 方法: 采用Diamonsil C₁₈(250 mm×4.6 mm,5 μm)色谱柱,以乙腈-0.5%磷酸溶液(18∶82)为流动相,流速1.0 mL·min^-1,检测波长308 nm,柱温30℃。 结果: 对香豆酸线性范围为0.0211~0.422 μg·mL^-1;平均回收率(n=6)为99.98%,RSD为1.2%。 结论: 该方法简便、准确,重复性好,可作为红花的质量控制方法。

Objective: To determine p-coumaric acid in Carthamus tinctoriu L. of various sources by HPLC. Methods: The determination of p-coumaric acid was performed on a Diamonsil C₁₈(250 mm×4.6 mm,5 μm)column;The mobile phase was acetonitrile-0.5% phosphoric acid(18∶82)at the flow rate of 1.0 mL·min^-1;The detecting wavelength was 308 nm and the column temperature was set at 30℃. Results: The linear range of p-coumaric acid was 0.0211-0.422 μg·mL^-1;The average recovery(n=6)was 99.98% with RSD of 1.2%. Conclusion: The method is simple and accurate with good reproducibility,and can be used to control the quality of Carthamus tinctorius.