RP-HPLC法测定天山花楸果实中苦杏仁苷、芦丁、金丝桃苷和橙皮苷的含量

目的: 建立同时测定维吾尔药材天山花楸果实中苦杏仁苷、芦丁、金丝桃苷和橙皮苷含量的RP-HPLC分析方法。 方法: 采用Shim-pack VP-ODS(250 mm×4.6 mm,5 μm)色谱柱,以乙腈(A)-0.05%磷酸水溶液(B)(三乙胺调pH 3.0)为流动相进行梯度洗脱(0~5 min,9%A→10%A;5~30 min,10%A→15%A;30~35 min, 15%A→17%A;35~50 min,17%A→21%A;50~60 min,21%A),流速1.0 mL·min^-1,检测波长215 nm,柱温25℃。 结果: 苦杏仁苷、芦丁、金丝桃苷和橙皮苷进样量分别在0.44~4.40,0.05~0.48,0.13~1.26,0.04~0.42 μg范围内,与色谱峰面积呈良好线性关系(r>0.9995,n=7);平均回收率(n=9)分别为99.5%,99.3%,100.1%,99.2%。 结论: 本法简便、准确,重复性好,可用于天山花楸药材的质量控制。

Objective: To develop an RP-HPLC method for the determination of amygdalin,rutin,hyperoside and hesperidin in fruit of Sorbus tianschanica Rupr. Methods: The analysis was carried out on a Shim-pack VP-ODS(250 mm×4.6 mm,5 μm)column.The mobile phase was composed of acetonitrile(A)and water containing 0.05% phosphoric acid(B)(adjusted to pH 3.0 with triethylamine)with gradient elution(0-5 min,9%A→10%A;5-30 min,10%A→15%A;30-35 min,15%A→17%A;35-50 min,17%A→21%A;50-60 min,21%A)and the flow rate was 1.0 mL·min^-1.The detection wavelength was 215 nm and the column temperature was 25℃. Results: The linear ranges of amygdalin,rutin,hyperoside and hesperidin were 0.44-4.40,0.05-0.48,0.13-1.26,0.04-0.42 μg respectively(r>0.9995,n=7).The average recoveries(n=9)were 99.5%,99.3%,100.1% and 99.2% respectively. Conclusion: The method is simple,accurate,and repeatable for simultaneous determination of amygdalin,rutin,hyperoside and hesperidin in fruit of Sorbus tianschanica Rupr.