HPLC同时测定21种淫羊藿中朝藿定C和淫羊藿苷的含量

目的: 建立同时测定淫羊藿药材中朝藿定C和淫羊藿苷含量的高效液相色谱方法。 方法: 采用Elite SinoChrom ODS-AP(250 mm×4.6 mm, 5 μm)色谱柱, 流动相为乙腈(A)-水(B), 梯度洗脱(0~8 min, 27%A;8~30 min, 27%A→29%A), 流速1 mL·min, 检测波长270 nm。 结果: 朝藿定C进样量在0.130~3.89 μg, 淫羊藿苷在0.0294~1.47 μg范围内呈良好线性关系(r=0.9999);朝藿定C和淫羊藿苷平均回收率(n=9)分别为103.9%, 100.0%;淫羊藿药材中朝藿定C和淫羊藿苷的含量分别为0.02%~7.80%, 0.01%~1.74%。 结论: 该方法简便、快速、准确, 重复性好, 可作为淫羊藿药材中朝霍定C和淫羊藿苷的含量测定方法。

Objective: To develop an HPLC method for simultaneous determination of epimedin C and icariin in 21 species of Epimedium. Methods: The samples were separated on Elite SinoChrom ODS-AP column(250 mm×4.6 mm,5 μm)by gradient elution with acetonitrile(A)-water(B)(0-8 min,27%A;8-30 min,27%A→29%A)as the mobile phase.The flow rate was 1 mL·min^-1,and the detection wavelength was 270 nm. Results: The linear ranges of epimedin C and icariin were 0.130-3.89 μg(r=0.9999),0.0294-1.47 μg(r=0.9999)respectively;The average recoveries(n=9)of epimedin C and icariin were 103.9%,100.0% respectively;The contents of epimedin C and icariin were 0.02%-7.80%,0.01%-1.74% respectively. Conclusion: This method is simple,quick,sensitive,reliable and reproducible.It is the best choice for the determination of epimedin C and icariin in Epimedium.