HPLC法测定金鸡片中3种生物碱的含量

目的: 建立HPLC法测定金鸡片中盐酸药根碱、盐酸巴马汀和盐酸小檗碱3种生物碱的含量。 方法: 选用WCX弱阳离子交换固相萃取小柱净化,采用Hvydro-RP 80A C₁₈(250 mm×4.6 mm,5 μm)色谱柱,以乙腈-0.05 mol·L^-1磷酸二氢钠溶液(磷酸调pH 2.8)(27∶ 73)为流动相,流速1.0 mL·min^-1,检测波长265 nm,柱温35 ℃。 结果: 盐酸药根碱、盐酸巴马汀、盐酸小檗碱进样量分别在4.94~49.44 ng(r=1.000)、4.36~43.60 ng(r=0.9999)和3.99~39.94 ng(r=0.9999)范围内线性关系良好;回收率(n=9)分别为98.32%,99.53%,98.35%。 结论: 该方法操作简便,准确性、重复性好,可用于金鸡片中盐酸药根碱、盐酸巴马汀和盐酸小檗碱的含量测定。

Objective: To establish an HPLC method for the determination of jatrorrhizine hydrochloride,palmatine hydrochloride and berberine hydrochloride in Jinji tablets. Methods: WCX weak cation SPE column was used to clean.The Hvydro-RP 80A C₁₈ column(250 mm×4.6 mm,5 μm)was used at 35 ℃ with acetonitrile-0.05 mol·L^-1 sodium dihydrogen phosphate(pH 2.8)(27∶ 73)as the mobile phase,1.0 mL·min^-1 as the flow rate,265 nm as the detective wavelength. Results: The linear ranges were 4.94-49.44 ng(r=1.000)for jatrorrhizine hydrochloride,4.36-43.60 ng(r=0.9999)for palmatine hydrochloride and 3.99-39.94 ng(r=0.9999)for berberine hydrochloride.The average recoveries(n=9)were 98.32%,99.53% and 98.35%,respectively. Conclusion: This method is simple,accurate and repeatable.It can be used for the determination of jatrorrhizine hydrochloride,palmatine hydrochloride and berberine hydrochloride in Jinji tablets.