乌灵胶囊的HPLC化学成分特征图谱研究

目的: 建立乌灵胶囊高效液相色谱化学成分特征图谱,为科学评价其质量提供可靠方法。 方法: 采用Kromasil 100-5 C₁₈ 柱(250 mm×4.6 mm,5 μm)色谱柱,以乙腈(A)-0.2%磷酸溶液(B)为流动相梯度洗脱(0~35 min,25% A→75% A),流速1.0 mL·min^-1 ,检测波长248 nm,柱温30 ℃。运用中药色谱指纹图谱相似度评价系统2004年版(国家药典委员会)进行分析。 结果: 构建了乌灵胶囊的特征图谱,提取分离得到其特征性成分5-甲基蜂蜜曲霉素。 结论: 该方法准确,重复性好,可作为乌灵胶囊的质控方法。

Objective: To establish the method of specific chromatogram analysis of chemical constituents by RP-HPLC with diode array detector(DAD) for the quality control of Wuling capsules. Methods: The specific chromatograms were obtained on an Kromasil 100-5 C₁₈ (250 mm×4.60 mm,5 μm) with the gradient elution solvent system composed of acetonitrile(A) and 0.2% solution of phosphoric acid(B)(0-35 min,25% A→75% A).The flow rate was 1.0 mL·min^-1,the column temperature was maintained at 30 ℃,and the detection wavelength was set at 248 nm.It was analyzed with the Estimating System of Similarity of 2004A Version(the Countrys Pharmacopeia Committee) on the Chinese Medicine Fingerprint Chromatogram. Results: The HPLC specific chromatogram of Wuling capsules was established.5-methylmellein was extracted as the characteristic component of Wuling capsules. Conclusion: The method with good precision and reproducibility is reliable for the quality control of Wuling capsules.