Lowry法测定寡肽的研究
Research of Lowry determination of oligopeptides
分类号:
出版年·卷·期(页码):2011,31 (4):0-0
DOI:
10.16155/j.0254-1793.2017.01.01
-----摘要:-------------------------------------------------------------------------------------------
目的: 探讨适合寡肽含量测定的方法。 方法: 通过测定海洋肽粉和大豆肽粉中寡肽含量,比较凯氏定氮法、双缩脲法、考马斯亮蓝G250法和Lowry法4种方法之间的差异。 结果: 4种方法测得的结果具有明显差异。 结论: Lowry法测得结果准确、可靠,灵敏度较高,易操作,较另外3种方法更适合于寡肽的含量测定。
-----英文摘要:---------------------------------------------------------------------------------------
Objective: To select suitable oligopeptide assay method. Methods: Determination of oligopeptide for the marine peptide powder and soybean peptide powder,compare the differences between the four kinds of methods,Kjeldahl method;biuret method;Coomassie Brilliant Blue G250 method and Lowry method. Results: The measured results in the four kinds of methods have significant differences. Conclusion: Lowry method measured results are accurate,reliable,and the method of high sensitivity,easy to operate,the relative other three kinds of method is more suitable for assay of oligopeptides.
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