顶空静态进样GC-MS分析羌活挥发性成分

目的: 建立顶空静态进样快速分析羌活挥发性成分的方法。 方法: 采用顶空静态进样技术提取羌活挥发性成分,对其进行气相色谱-质谱(GC-MS)结合保留指数双重分析。 结果: 通过GC-MS及保留指数双重定性,鉴定出羌活中24个挥发性化学成分,其中α-蒎烯、β-蒎烯、3-蒈烯、D-柠檬烯4种成分约占羌活挥发性化学成分的82.6%。 结论: 顶空静态进样技术结合GC-MS及保留指数定性,可以方便、快捷地提供羌活中挥发性成分的化学信息。

Objective: To establish a rapid analytical method for volatile components in rhizoma et radix notopterygii. Methods: The volatile components were extracted by head-space injection technique and analyzed by gas chromatography-mass spectrometry (GC-MS) combined with Kovats retention indices. Results: Among the volatile components that detected,36 compounds were identified by GC-MS combined with Kovats retention indices;the content of four volatile compounds,including α-pinene,β-pinene,3-carene and D-limonene,can reach up to 81% of the total. Conclusions: GC-MS and Kovats retention indices combined with static headspace injection technique can be used as a rapid,direct and convenient analytical method for the volatile components in Chinese herbal medicine.