离子对高效液相色谱荧光法测定人血浆中顺式阿曲库铵的浓度

目的: 建立反相离子对高效液相色谱荧光法测定人血浆中顺式阿曲库铵浓度。 方法: 酸化的血浆样品采用乙腈沉淀蛋白处理,取上清液进样分析。色谱柱为Agilent Eclipse C₁₈(150 mm×4.6 mm,5 μm),流动相为1.5%三氟乙酸-乙腈(55∶ 45),流速0.5 mL·min^-1,荧光的激发波长为280 nm,发射波长为320 nm。 结果: 顺式阿曲库铵在12.4~4960.0 ng·mL^-1范围内线性关系良好(r=0.9998),检测限为1.24 ng·mL^-1,平均提取收率为85.1%~88.5%,平均方法回收率为98.3%~102.1 %,日内、日间RSD均＜3.4％。 结论: 本方法操作简便、灵敏度高、专一性强、结果可靠,可满足临床检测和药代动力学研究的需要。

Objective: To establish iron-pair HPLC-fluorescence method for determination of cisatracurium in human plasma. Methods: Acidified plasma samples were pretreated with acetonitrile to precipitate protein and the supernatant liquid obtained from the plasma by centrifuge was subjected to chromatographic analysis.The separation was performed on a reversed-phase Agilent Eclipse C₁₈ (150 mm×4.6 mm,5 μm),the mobile phase was made up of 1.5% trifluoroacetic acid-acetonitrile (55∶ 45) at a flow rate of 0.5 mL·min^-1,the excitation and emission wavelengths are 280 nm and 320 nm,respectively. Results: Calibration curves for cisatracurium was 12.4-4960.0 ng·mL^-1(r=0.9998).The limit of detection was 1.24 ng·mL^-1.The average extraction recovery was 85.1%-88.5%,and the average method recovery was 98.3%-102.1%.The intra-day and inter-day RSDs were both less than 3.4%. Conclusion: The proposed method is simple,sensitive,accurate and reliable for the need of clinical detection and the pharmacokinetic research.