UPLC法测定通塞脉微丸中绿原酸、阿魏酸、甘草苷、肉桂酸的含量

目的: 建立同时测定通塞脉微丸中绿原酸、阿魏酸、甘草苷、肉桂酸4种成分的超高效液相色谱(UPLC)分析方法。 方法: 采用Waters AcquityUPLC系统,AcquityUPLC BEH C₁₈ (50 mm×2.1 mm,1.7 μm)色谱柱,以乙腈-0.1%甲酸水为流动相,梯度洗脱,双波长检测(λ₁=276 nm、λ₂=327 nm),柱温35 ℃。 结果: 绿原酸、阿魏酸、甘草苷、肉桂酸在16 min内即达到基线分离,在测定的浓度范围内线性关系良好(r≥0.9997),各成分的平均回收率在95.1%~98.2%,RSD<4.0%。 结论: UPLC分离效果及重复性好,且快速、简便,可作为通塞脉微丸的质量控制方法。

Objective: To establish a ultra performance liquid chromatography(UPLC) method for simultaneous determination of 4 components—chlorogenic acid,ferulic acid,liquiritin and cinnamic acid in Tongsaimai pellets. Methods: The analyses were performed on a Waters AcquityUPLC system equipped with a binary solventmanager,a samplermanager,a column compartment and a PDA detector,connected to Waters Empower 2 software.An AcquityUPLC BEH C₁₈ column (50 mm×2.1 mm,1.7 μm)was used for all analyses.The investigated compounds were separated with gradient mobile phase consisting of acetomitrile-0.1% formic acid.Chlorogenic acid,ferulic acid,liquiritin and cinnamic acid were determined by dual wavelength of 276 nm(λ₁) and 327 nm(λ₂),and column temperature 35 ℃. Results: Four components could be well separated at the baseline in 16 min.The average recovery was 95.1% to 98.2% with RSD<4.0%. Conclusion: The developed UPLC method is simple,sensitive and accurate with good repeatability,which is available for quality control of Tongsaimai pellets.