气相色谱法同时测定吐温80中7种脂肪酸亲油基的含量

目的: 建立用GC测定吐温80中亲油基含量的方法,为吐温80使用适宜性研究奠定基础。 方法: 采用GC法,选用DB-1毛细管色谱柱,柱温185 ℃,进样口温度250 ℃,检测器温度250 ℃。 结果: 吐温80中7种亲油基衍生的脂肪酸甲酯(油酸甲酯、亚油酸甲酯、硬脂酸甲酯、肉豆蔻酸甲酯、棕榈油酸甲酯、棕榈酸甲酯、亚麻酸甲酯)在测定范围(1.8~35.5 mg;0.4~2.1 mg;0.5~4.1 mg;0.2~1.2 mg;0.6~2.9 mg;0.5~2.7 mg;0.6~3.0 mg)内有良好线性关系;油酸甲酯的平均回收率为100.7%。 结论: 本方法简单,稳定易行,可控性强,重复性好,可用于控制吐温80的质量。

Objective: An GC method for determination the quantitative analysis of hydrophobic group in Tween-80 was developed to provide the theoretical basis for Tween-80. Methods: DB-1 capillary column was used,column temperature was 185 ℃,injection port temperature of GC was set at 250 ℃,detector temperature was set at 250 ℃. Results: Methyl oleate,methyl linoleate,methyl stearate,methyl myristate,methyl palmitoleate,methyl palmitate,methyl linolenate assumed fine linear relationship at the sample size of 1.8-35.5 mg,0.4-2.1 mg,0.5-4.1 mg,0.2-1.2 mg,0.6-2.9 mg,0.5-2.7 mg,0.6-3.0 mg,respectively;The average recoveries of the method was 100.7%. Conclusion: The method is reliable,and can be used for quality control of Tween-80.