HPLC法测定蒲葵药材中6种组分含量

目的: 建立HPLC法同时测定蒲葵中荭草苷、异荭草苷、牡荆苷、异牡荆苷、异鼠李素-3-O-β-D-葡萄糖苷、苜蓿素含量,并比较不同产地不同部位蒲葵中6种组分的含量。 方法: 采用Agilent TC-C₁₈(4.6 mm×250 mm,5 μm)色谱柱;以甲醇(B)-0.05%醋酸溶液(A)为流动相,梯度洗脱(0~25 min,B: 33%→45%;25~40 min,B:45%→80%;40~45 min,B:80%→95%;45~50 min,B:95%→95%;50~60 min,B:95%→33%);流速0.800 mL·min^-1;检测波长340 nm,柱温30 ℃。 结果: 6个成分的检测范围分别为荭草苷:8.59~550.0 μg·mL^-1(r=0.9997)、异荭草苷:9.37~600.0 μg·mL^-1(r=0.9999)、牡荆苷:8.59~550.0 μg·mL^-1(r=0.9998)、异牡荆苷:8.59~550.0 μg·mL^-1(r=0.9995)、异鼠李素-3-O-β-D-葡萄糖苷:7.81~500.0 μg·mL^-1(r=0.9999),苜蓿素:8.59~550.0 μg·mL^-1(r=0.9999);平均回收率(n=3)分别为102.9%(RSD=4.6%),102.3%(RSD=2.3%),93.3%(RSD=2.1%),95.8%(RSD=2.7%),101.7%(RSD=1.8%),99.2%(RSD=2.5%)。 结论: 本方法具有良好的重复性,为评价蒲葵药材的质量提供了分析方法。不同产地不同部位的含量存在显著性差异。

Objective: To develop an HPLC method to determinate the proportion of orientin,isoorientin,vitexin,isovitexin,isorhamnetin-3-O-β-D-glucoside,tricin of different parts in Livistona chinensis from different origins. Method: The separation was performed on an Agilent TC-C₁₈(4.6 mm×250 mm,5 μm) with a gradient elution(0-25 min,B:33%→45%;25-40 min,B:45%→80%;40-45 min,B:80%→95%;45-50 min,B:95%→95%) composed of methanol(B) and 0.05% acetic acid(A).The column temperature was set at 30 ℃ with flow rate of 0.8 mL·min^-1.The detective wavelength was set at 340 nm. Results: The calibration curve was linear over the concentration of 8.59-550.0 μg·mL^-1 for orientin(r=0.9997);9.37-600.0 μg·mL^-1 for isoorientin(r=0.9999);8.59-550.0 μg·mL^-1for vitexin(r=0.9998);8.59-550.0 μg·mL^-1 for isovitexin(r=0.9995);7.81-500.0 μg·mL^-1 for isorhamnetin-3-O-β-D-glucoside(r=0.9999);8.59-550.0 μg·mL^-1 for tricin(r=0.9999).The average recovery of six components were 102.9%,102.3%,93.3%,95.8%,101.7%,99.2% and the RSDs were 4.6%,2.3%,2.1%,2.7%,1.8% and 2.5%,respectively. Conclusion: The method is reproducible,and can be used for the quality control of Livistona chinensis.The yields of the six components in different parts of different origins exhibite significant differences.