液相色谱－串联质谱法同时测定穿心莲中敌克松、甲基托布津、甲萘威和敌百虫的农药残留量

目的: 建立测定穿心莲叶中常用的4种农药残留量的方法,更好地控制药材的质量。 方法: 以灭蚜威为测定内标,样品经乙腈提取,采用Atlantics d C₁₈柱分离,液相色谱－串联质谱联用(LC—MS/MS)方法测定穿心莲药材中常用的4种农药残留量。 结果: 4种农药检测限在0.003~0.0093 mg·kg^-1之间,回收率在83%~112％之间,回收率相对标准偏差均低于6％。 结论: 方法简便、灵敏、重现性好,能满足同时测定4种农药在穿心莲药材中的残留量。

Objective: To establish a reliable method for simultaneous determination of four residual pesticides(fenaminosulf,thiophanate-methyl,carbaryl and trichlorphon)in Andrograghis paniculata (Burm.F.)Nees. Methods: The analytes were extracted with acetonitrile with macarbam as internal standard,then separated on an Atlantics d C₁₈ column.Detection was performed on LC—MS/MS system with ESI resources in positive ion acquisition mode and qualified in multiple reaction monitoring (MRM)mode. Results: The LODs of four pesticides were between 0.003-0.0093 mg·kg^-1.Recoveries were between 83%-112%,and the RSDs of recoveries were all less than 6%. Conclusion: The method established in this study is simple,sensitive and reproductive,which can be applied to the analysis of pesticide residues in Andrograghis paniculata (Burm.F.)Nees.