纳米银凝胶在体内分布特性的初步研究

目的: 研究纳米银妇女外用抗菌凝胶在兔体内的吸收和分布情况,以了解纳米银凝胶是否具有在体内迁移和蓄积的特性。 方法: 将成年新西兰雌兔经阴道注入纳米银凝胶,给药剂量为0.575 g · kg^-1 · d^-1,连续给药1个疗程后,在预定时间点解剖并取各动物血液和主要组织器官,利用超高压微波消解系统消解这些组织器官,再用原子吸收光谱仪检测消解液中的银含量。 结果: 在给药1个疗程后的8 h,纳米银在试验组各组织中的银含量达高峰,与对照组各脏器中银含量进行比较,结果有显著性差异(P<0.05)。其中阴道是纳米银主要的分布器官,其银含量高于其他组织达3倍以上。1周以后各组织中的银含量均大幅度下降,到1个月和3个月后,各试验组脏器中的银含量与对照组无显著性差异(P>0.05)。 结论: 纳米银凝胶中的纳米银可以通过血液循环系统迁移,并在兔体内各主要脏器肝、肾、脾、子宫、阴道等中分布,其中阴道是其主要的分布器官。在采用5倍临床给药剂量1个疗程的情况下,纳米银凝胶1周后在这些脏器中无明显蓄积。在下一步的研究中还需进一步了解纳米银凝胶在体内的状态、进入血液系统的方式和对各部位细胞的影响。

Objective: To investigate the absorption and distribution of nano silver gel for external use in the rabbit body and to analyze whether the nano silver gel have the character of translocation and accumulation. Methods: Adult female New Zealand rabbits were injected with nano silver gel at 0.575 g · kg^-1 · d^-1 via vagina,and then they were sacrificed at predetermined time points after a period of treatment.The main organs of the experimental animals were harvested for silver content analysis by using the extra high pressure microwave and the atom absorption spectrum instrument. Results: Eight hours after the last dose administration,the nano silver content of test group were remarkably increase compared to the control group(P<0.05).The vagina was a main distribution organ of nano silver,in which the silver content was three times more than in others.The silver content in each organ significantly decreased after a week,and had not remarkable difference in each organ after 1 month and 3 months post-treatment compared to the control groups(P>0.05). Conclusion: The nano silver gel can move through the blood circulation system and distribute in liver,kidney,spleen,womb and vagina etc.Under the condition of adopting 5 times clinical doses after a period of treatment,the nano silver gel has not obviously accumulation in these organs after a month.It still need further study to investigate the nano silver gel about the state in body,the way into blood system,and the influence to cells.