RP-HPLC测定硝苯地平片的含量

目的: 采用高效液相色谱法测定硝苯地平片含量。 方法: 采用Symmetry C₁₈色谱柱(4.6 mm×250 mm_,5 μm), 以水-乙腈-甲醇(30∶ 35∶ 35)为流动相,流速1.0 mL·min^-1,检测波长235 nm,进样量20 μL。 结果: 硝苯地平线性范围为 40~140 μg·mL^-1 (r=0.9996),最小检测量为7.7 ng。 结论: 本法精密度好,结果准确、可靠、灵敏,可用于硝苯地平片的含量测定。

Objective: To develop an HPLC method for the determination of content of nifedipine. Methods: The chromatography was performed on Symmetry C₁₈ column(4.6 mm×250 mm,5 μm) water,acetonitrile,and methanol(30∶ 35∶ 35) as the mobile phase at a flow rate of 1.0 mL·min^-1 and the absorbance was monitored at 235 nm.The injection volume was 20 μL. Results: The calibration curves of Nifedipine was linear in the range of 40-140 μg·mL^-1 (r=0.9996).The limit of detection was 7.7 ng. Conclusion: The method is accurate,credible and sensitive.It can be used for controlling the quality of nifedipine tablets.