RP-HPLC法测定余甘子中诃黎勒酸、没食子酸、粘酸-2-O-没食子酸酯的含量

目的: 建立RP-HPLC法同时测定余甘子(Phyllanthus emblica L.干燥成熟果实)中诃黎勒酸、没食子酸、粘酸-2-O-没食子酸酯含量。 方法: 采用Diamonsil C₁₈柱(250 mm×4.6 mm,5 μm),以甲醇-0.2%醋酸水溶液(19∶ 81,v/v)为流动相;检测波长为275 nm;流速为1 mL·min^-1;柱温为30 ℃。 结果: 诃黎勒酸、没食子酸、粘酸-2-O-没食子酸酯线性范围分别为0.235~1.176 μg、0.185~0.924 μg、0.418~2.09 μg;平均回收率(n=6)分别为101.5%,99.20%,101.5%。 结论: 建立的方法简便,重现性好,可用于余甘子中诃黎勒酸、没食子酸、粘酸-2-O-没食子酸酯的含量测定。

Objective: To establish an RP-HPLC method for the assaying of the content of chebuligic acid,gallic acid,mucic acid 2-O-gallate in Fructus Phyllanthi. Method: The analytes were separated on a Diamonsil C₁₈ column (250 mm×4.6 mm,5 μm) and column temperature was 30 ℃.The mobile phase was the system of methanol-0.2% aqueous acetic acid (19∶ 81,v/v) with flow rate of 1 mL·min^-1,the UV detection was set at 275 nm. Results: The analytes were well separated with the linear range of 0.235-1.176 μg for chebuligic acid,0.185-0.924 μg for gallic acid,and 0.418-2.09 μg for mucic acid 2-O-gallate. The average recoveries(n=6) of chebuligic acid,gallic acid and mucic acid 2-O-gallate were 101.5% (RSD=2.4%),99.20% (RSD=2.6%),101.52% (RSD=2.0%),respectively. Conclusion: The method established is simple and reproducible,and can be used for determination of chebuligic acid,gallic acid,and mucic acid 2-O-gallate in Fructus Phyllanthi.