超高效液相色谱法测定苏南山肚痛丸中芍药苷的含量

目的: 建立用超高效液相色谱(UPLC)法快速测定苏南山肚痛丸中芍药苷含量。 方法: 采用Acquity BEH C₁₈(2.1 mm×50 mm,1.7 μm)色谱柱,以乙腈-0.1%磷酸(15∶ 85)为流动相,流速0.21 mL·min^-1,检测波长230 nm,柱温25 ℃,进样体积2 μL,采集频率20 Hz。利用Acquity Waters system进行含量测定。 结果: 样品中芍药苷在4.5 min内完全分离并对其进行了含量测定,芍药苷进样量在0.026~0.264 μg范围内与峰面积呈良好的线性关系(r=0.9999),平均回收率(n=6)为102.2%(RSD=0.4%)。 结论: UPLC法在不影响分离效果的情况下大大提高了芍药苷分析速度,同时减少了溶剂消耗。该研究表明UPLC法可作为传统HPLC法的一种更为方便、可行的替代方法。

Objective: To establish a ultra-performance liquid chromatography(UPLC) method for determination of paeoniflorin in Sunanshandutong pills. Methods: The separation was performed on Acquity BEH C₁₈(2.1 mm×50 mm,1.7 μm) column;The mobile phase was acetonitrile-0.1% Phosphoric acid(15∶ 85) at a flow rate of 0.21 mL·min^-1;The detection wavelength was 230 nm,and the column temperature was 25 ℃;Injection volume was 2 μL;Collection frequency was 20 Hz.Acquity Waters system was used for the assay. Results: Paeoniflorin was completely separated and detected in 4.5 minutes;Linear range was 0.026-0.264 μg(r=0.9999);Recovery(n=6) of method was 102.2%,RSD was 0.4%. Conclusion: The method is sensitive,accurate,precise,repeatable and suitable for determination of paeoniflorin in Sunanshandutong pills.Comparison to HPLC,UPLC obtain a faster analysis and less solvent consumption without reducing the efficiency.