多波长高效液相色谱法同时测定消肿止痛酊中3种有效成分的含量

目的: 利用多波长高效液相色谱法,建立消肿止痛酊中3种有效成分(栀子苷、丹皮酚、樟脑)的含量测定方法,考察该产品在有效期内不同生产日期的内在质量。 方法: 采用Waters SunFire C₁₈(4.6 mm×250 mm,5 μm)色谱柱,柱温30 ℃;甲醇(A)-水(B)为流动相,以1 mL·min^-1流速梯度洗脱;检测波长:238 nm(0~14 min,检测栀子苷)、274 nm(15~32 min,检测丹皮酚)、288 nm(33~60 min,检测樟脑)。 结果: 在60 min内消肿止痛酊中栀子苷、丹皮酚、樟脑3种有效成分被完全分离,峰面积与其浓度呈良好的线性关系(r=0.9999);加样回收率(n=6):栀子苷为100.9%(RSD=0.6%),丹皮酚为100.2%(RSD=1.4%),樟脑为100.6%(RSD=1.0%)。 结论: 本方法操作简单,结果准确,为有效控制消肿止痛酊质量,提升质量标准,提供了一种可靠的检测方法。

Objective: To establish an HPLC with multi-wavelength detection method for simultaneous determination of geniposide,paeonol and camphor in Xiaozhong Zhitong tincture,and the quality of the tincture produced in different date was investigated using this developed method. Methods: The samples were separated on a Waters SunFire C₁₈(4.6 mm×250 mm,5 μm) column,by a gradient elution using methanol(A) and water(B) as mobile phase at a flow rate of 1.0 mL·min^-1 at 30 ℃;The eluate was detected by multiple wavelength:238 nm for geniposide(0-14 min),274 nm for paeonol(15-32 min),288 nm for camphor(33-60 min). Results: The complete separation was obtained within 60 min for the three compounds(geniposide,paeonol and camphor);Three regression equations showing linear relationships between peak-area and content of each compound were obtained.The average recoveries(n=6) of the three compounds were 100.9%(RSD=0.6%),100.2%(RSD=1.4%),100.6%(RSD=1.0%),respectively. Conclusion: This method is simple,accurate,and provides a reliable detection methods for valid control of the product quality and improvement of quality standard.