HPLC测定康泰胶囊中5种化合物的含量

目的: 建立测定康泰胶囊(防风、白芍、木香)含量的方法。 方法: 采用高效液相色谱法在同一色谱条件 下对制剂中升麻苷、芍药苷、5-O-甲基维斯阿米醇苷、木香烃内酯及去氢木香烃内酯的含量进行测定。 结果: 升麻苷、芍药苷、5-O-甲基维斯阿米醇苷、木香烃内酯、去氢木香烃内酯的线性范围分别为13.15~236.74 ng(r=0.9999),246.00~4428.00 ng(r=0.9995),35.12~632.16 ng(r=0.9993),35.00~630.00 ng(r=0.9997),27.20~489.60 ng(r=0.9996);平均回收率(n=9)分别为101.0%,100.9%,99.79%,101.0%,100.2%。 结论: 该方法简便、快速、准确,重现性好,可作为康泰胶囊质量控制的方法。

Objective: To establish a method for the simultaneous determination of prim-O-glucosylcimifugin,paeoniflorin,5-O-methylvisammioside,costunolide and dehydrocostuslactone in Kangtai capsules. Methods: The HPLC system consisted of a Kromasil 100-5C₁₈ (250 mm×4.6 mm,5 μm) column,acetonitrile-0.1% phosphoric acid as mobile phase with gradient elution at a flow rate of 1.0 mL·min^-1,and detection wavelength of 220 nm. Results: The linear ranges of prim-O-glucosylcimifugin,paeoniflorin,5-O-methylvisammioside,costunolide and dehydrocostuslactone were 13.15-236.74 ng(r=0.9999),246.00-4428.00 ng(r=0.9995),35.12-632.16 ng(r=0.9993),35.00-630.00 ng(r=0.9997),27.20-489.60 ng(r=0.9996),respectively;The average recoveries(n=9) were 101.0%,100.9%,99.79%,101.0%,100.2%,respectively. Conclusion: The method is simple,quick,accurate and reproducible,and can be used for the quality control of Kangtai capsules.